Genetic diversity of Dimocarpus longan in China revealed by AFLP markers and partial rbcL gene sequences |
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| Affiliation: | 1. Fujian Agriculture and Forestry University, Jinshan, Fuzhou, Fujian 350002, P.R. China;2. Japan International Research Center for Agricultural Sciences, Okinawa Subtropical Station, Maezato 1091-1, Ishigaki, Okinawa 907-0002, Japan;1. Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS 91501-970, Brazil;2. Department of Botany, Federal University of Santa Maria, Santa Maria, RS, Brazil;3. Institute of Pathophysiology and Immunology, Medical University of Graz, 8010 Graz, Austria;5. Institute of Cell Biology, Histology, and Embryology, Medical University of Graz, 8010 Graz, Austria;6. Institute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria;4. National Institute for Health and Welfare, Biomedicum, FI-00290 Helsinki, Finland;1. Pharmacognosy Department, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt;2. Tissue Culture Unit, Plant Genetic Resources Department, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-Matarya, Cairo, Egypt;3. Pharmacognosy Department, Faculty of Pharmacy, Assiut University, 71515 Assiut, Egypt;1. Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy;2. Department of Food and Drug, University of Parma, Parma, Italy;3. NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany;1. College of Oceanology, Harbin Institute of Technology (Weihai), Weihai 264209, PR China;2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China |
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| Abstract: | ![]()
Amplified fragment length polymorphism (AFLP) and partial rbcL gene sequencing were used to investigate genetic diversity among various longan (Dimocarpus longan Lour) accessions as well as a presumed closely related species Dimocarpus confinis How et Ho and litchi (Litchi chinensis Sonn). No significantly shared AFLP fragment was found between the three species, indicating that D. confinis and litchi are very far in genetic distance from any longan accession studied. Partial rbcL sequences of 501 bp from the first coding site in these species were obtained, which revealed several substitutes. One such DNA base pair substitute resulted in an amino acid difference between longan and litchi. Furthermore, another 4 bp resulted in a two amino acid difference between longan and D. confinis, which was consistent with AFLP results and indicated that D. confinis should be excluded from the longan genus, Dimocarpus. Within the longan species, no DNA substitute was found. Using nine primer combinations, a total of 66 AFLP markers were obtained from 41 longan accessions. One non-Chinese longan accession ‘Miaoqiao’ was distinctly different from all other longan cultivars collected in China, indicating that more genetic resources of longan might be collected also from longan production regions outside of China. AFLP markers might be developed to identify longan cultivars as well as expedite progeny screening in breeding programs of this perennial fruit tree. |
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