In vitro adventitious rooting of Cornus florida microshoots |
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| Institution: | 1. Department of Entomology and Plant Pathology, The University of Tennessee, 205 Ellington Plant Science Building, Knoxville, TN 37996-4560, USA;2. Department of Plant Sciences, The University of Tennessee, Knoxville, TN 37996-4561, USA;3. Department of Botany, The University of Tennessee, Knoxville, TN 37996-1100, USA;1. Department of Land, Air and Water Resources, University of California, Davis, CA 95616, USA;2. Institute for Evolutionary Biology and Environmental Studies, University of Zurich, 8057 Zurich, Switzerland;3. Plant-Soil-Interactions, Agroscope Institute for Sustainability Sciences, 8046 Zurich, Switzerland;4. Plant-Microbe Interactions, Institute of Environmental Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands;1. College of Agriculture, Henan University of Science and Techenology, Luoyang 471003, China;2. College of Life Science, Shaoxing University, Shaoxing 312000, China;3. College of Life Science, Anhui Agricultural University, Hefei 230036, China;4. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;1. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand;2. Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand;3. Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;4. Natural Product Research Unit, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand;1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;2. Research Center of Agricultural Environment and Resources, Jilin Academy of Agricultural Sciences, Changchun 130033, China |
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| Abstract: | Cornus florida L. (flowering dogwood) has been successfully micropropagated, but low rooting of microshoots makes the system inefficient. This study was conducted to increase rooting efficiency of flowering dogwood microshoots over that previously achieved. Microshoots originating from acclimatized axillary and nodal bud stock cultures were excised and treated with different concentrations and combinations of various auxins including indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA). Effect of microshoot age on rooting efficiency was also examined. Of the auxins tested, maximum rooting was observed with 4.4 μM IBA. The age of microshoot explants had a significant effect on rooting. Five to seven-week-old microshoots treated continuously with 4.9 μM IBA in Woody plant medium (WPM) consistently had the best and most consistent rooting efficiency (about 83%). |
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