Epidemiology and genetic characterization of BVDV,BHV-1, BHV-4, BHV-5 and
Brucella spp. infections in cattle in Turkey |
| |
Authors: | Muhammet Eren ASLAN Ahmet Kursat AZKUR Serkal GAZYAGCI |
| |
Institution: | 1)Department of Virology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale 71450, Turkey;2)Department of Internal Medicine, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale 71450, Turkey |
| |
Abstract: | The aim of the study was to determine the epidemiological data of bovine viral diarrhea
virus (BVDV), bovine herpesvirus-1 (BHV-1), bovine herpesvirus-4 (BHV-4), bovine
herpesvirus-5 (BHV-5) and Brucella–associated cattle that were previously
reported to have abortion and infertility problems in Ankara, Corum, Kirikkale and Yozgat
provinces, Turkey. Whole blood and sera samples were obtained from 656 cattle, and
antibodies against Brucella spp. were detected in 45 (6.86%) and 41
(6.25%) animals by Rose Bengal plate and serum tube agglutination tests, respectively. The
seropositivity rates against BVDV, BHV-1 and BHV-4 were 70.89%, 41.3% and 28.78%,
respectively. RT-PCR and PCR were performed to detect RNA and DNA viruses in blood
samples, respectively. The BVDV 5′-untranslated region and BHV-1 gB gene detected in this
study were phylogenetically analyzed. The BVDV strains analyzed in this study were closely
related to those previously reported from Turkey. The nucleotide sequence from the BHV-1
strain detected in this study is the first nucleotide sequence of BHV-1 circulating in
this area of Turkey deposited in the GenBank. The presence of Brucella
spp. and prevalence of BHV-1, BHV-4 and BVDV in cattle should be further investigated
throughout these regions. |
| |
Keywords: | abortion agent cattle phylogenetic analysis seroprevalence |
|
|