Biochemical changes in micropropagated grape (Vitis vinifera L.) plantlets due to arbuscular-mycorrhizal fungi (AMF) inoculation during ex vitro acclimatization |
| |
| Institution: | 1. Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India;2. Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Kadiri 515 591, Andhra Pradesh, India;1. Institute of Agricultural Biology and Agrobiotechnology, CNR, UOS Pisa, Pisa, Italy;2. Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy;3. Interdepartmental Research Center “Nutraceuticals and Food for Health,” University of Pisa, Pisa, Italy;1. Department of Plant Production, Faculty of Food & Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia;2. Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia;1. College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China;2. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, 510640, China;3. Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove 50003, Czech Republic;1. Council for Agricultural Research and Economics- Research Centre for Genomics and Bioinformatics (CREA-GB), via San Protaso 302, 29017, Fiorenzuola d''Arda, PC, Italy;2. Department for Sustainable Food Process, Research Centre for Nutrigenomics and Proteomics, Università Cattolica del Sacro Cuore, Piacenza, Italy;3. Department of Agronomy, Food, Natural Resources, Animals and Environment, Università di Padova, Padova, Italy;4. Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy;5. Department of Agriculture and Forest Sciences, University of Tuscia, Viterbo, Italy;1. Université de Carthage, Institut National Agronomique de Tunisie, LR13AGR01, Laboratoire des Sciences Horticoles, 43 Ave Charles Nicolle, 1082, Tunis, Mahrajène, Tunisia;2. Université du Littoral Côte d′Opale, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), UR4492, SFR Condorcet FR CNRS 3417, 50 rue Ferdinand Buisson, 62228, Calais, France |
| |
| Abstract: | Improved establishment of mycorrhizal tissue culture derived plantlets during acclimatization (Stage IV) is commonly attributed to the enhanced vegetative growth as a resultant of different morphological and in vivo changes. These changes are early and better cuticle development, high biomass accumulation, enhanced physiological changes, improved mineral nutrition, especially phosphorus and micronutrients, etc. However, improvement in establishment of micropropagated plantlets during acclimatization may not only be limited to these mechanisms. In the present investigation, biochemical status of micropropagated grape plantlets in response to six single and a mixed strains of arbuscular-mycorrhizal fungi (AMF) during hardening were studied under glasshouse conditions. The histochemical studies revealed that the mycorrhizal inoculation resulted in accumulation of different biochemicals in the plant system such as chlorophyll, carotenoids, proline, phenol and enzymes like polyphenol oxidase (PPO) and nitrate reductase (NR). The mycorrhizal plantlets showed enhanced survival and improved tolerance against stresses experienced during weaning phase. The mycorrhizal plants also exhibited improved physiological and nutritional status and had higher relative water content (RWC) and photosynthetic rate. These plantlets also accumulated higher N, P, Mg and Fe concentrations, which may primarily be as a result of biochemical changes brought about by mycorrhizal association. Mycorrhizal plantlets also showed better hardening under glasshouse conditions. The result suggests that the biochemical changes brought about by mycorrhization were helpful in mitigating different stresses experienced by the tissue culture plants during hardening, which determine their performance later in field. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
