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Locust bean gum (LBG) as a gelling agent for plant tissue culture media
Affiliation:1. Postharvest Technology Research Laboratory, South African Research Chair in Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Stellenbosch 7602, South Africa;2. Plant Science Laboratory, Cranfield University, Bedfordshire MK43 0AL, United Kingdom;3. Citrus Research International, Department of Horticultural Science, Stellenbosch University, Stellenbosch 7602, South Africa;4. Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch 7602, South Africa;5. Department of Environmental Science and Technology, School of Applied Sciences, Cranfield University, Bedfordshire MK43 0AL, United Kingdom;6. MeBioS Division, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, B-3001 Leuven, Belgium;1. Laboratory of Vegetable Production, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos str., 11855 Athens, Greece;2. Laboratory of Plant Physiology and Morphology, Agricultural University of Athens, 75 Iera Odos str., 11855 Athens, Greece;1. Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, 730070, China;2. Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, 430064, China;1. COMSATS Institute of Information Technology, Department of Environmental Sciences, 22060 Abbottabad, Pakistan;2. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008 Nanjing, China
Abstract:Locust bean gum (LBG) is a natural hydrocolloid extracted from the seeds of carob tree (Ceratonia siliqua L.). This work describes the successful use of LBG as a gelling agent in combination with agar for shoot multiplication and rooting of carob tree and Iberian rose shoots. Its presence did not affect the multiplication rate of both species. The rooting frequency of carob shoots was even significantly increased in the presence of 5 g of LBG plus 4 g of agar to the medium compared to medium solidified with 9 g of agar. Iberian rose shoots rooting was not influenced by the addition of this gum to the rooting medium. Results obtained show that LBG can be used in combination with agar in culture medium as a gelling agent without negative effect on plant material and with the advantage of reduced medium costs.
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