基于SRAP标记构建野生香菇核心种质库

以95份野生香菇(Lentinula edodes)种质和1份栽培种质为材料,在利用相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)标记开展遗传多样性分析的基础上,采用属性约简启发式算法和逐步聚类位点优先取样法分别构建核心种质。两种方法分别得到35份(Core 1)和29份(Core 2)种质,其核心种质保留比分别为36.5%和30.2%,位点保留比分别为100%和94.7%。基于有效等位基因数,Nei’s基因多样性指数,香农信息指数等四个参数对两组核心种质进行t测验,结果显示两组核心样本均能很好的代表原始种质的遗传多样性,但Core1具有更高的位点保留比例且地理来源更加广泛,确定为代表96个原始菌株的核心种质库。

Constructing Core Collections of ChineseWild Lentinula edodes Strains Basedon SRAP Markers

Genetic diversity among 95 wild strains and one cultivar of Lentinula edodes obtained from Chinese sources was analyzed using sequence-related amplified polymorphism (SRAP) markers.Core collections for the 96 strains were then constructed by employing the modified heuristic parsimony algorithm (Core 1) and the allele preferred sampling method (Core 2).Using the two strategies, 35 strains were assigned to Core 1 and 29 strains to Core 2, accounting for 36.5% and 30.2% of total samples tested respectively, and the loci retention ratios of the two core collections were 100% and 94.7%, respectively.Use of the t test to analyze the differences in the observed number of alleles, the effective number of alleles, Nei's genetic diversity index and the Shannon information index between the two core collections and the original collection revealed that both core collections were representative of the original germplasm.However, since the loci retention values and geographical distribution of Core 1 strains were greater than those in Core 2, the former was designated the core collection for the original 96 L.edodes strains.