| 猴头菌子实体提取物对人肺癌细胞SPCA-1的抑制作用 |
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| 引用本文： | 汪雯翰,贾薇,张劲松,杨海芮,杨焱,朱丽娜. 猴头菌子实体提取物对人肺癌细胞SPCA-1的抑制作用[J]. 食用菌学报, 2017, 0(1): 89-92. DOI: 10.16488/j.cnki.1005-9873.2017.01.016 |
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| 作者姓名： | 汪雯翰 贾薇 张劲松 杨海芮 杨焱 朱丽娜 |
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| 作者单位： | 1. 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验,上海 201403;2. 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验,上海 201403;石河子大学生命科学学院,新疆石河子 832000 |
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| 基金项目： | 上海市科技兴农种业项目[沪农科种字(2016)第1-12号]资助 |
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| 摘 要： | 猴头菌(Hericium erinaceus)子实体的醇提物用不同极性有机溶剂分部提取,得到石油醚、氯仿、乙酸乙酯和正丁醇分部,其中仅石油醚分部能抑制SPCA-1细胞增殖和促进其释放ROS(reactive oxygen species),对石油醚分部的进一步研究发现石油醚分部可诱导SPCA-1细胞早期凋亡和降低G_0/G_1期细胞数量。
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| 关 键 词： | 石油醚分部 抑制率 细胞凋亡 细胞周期 |
Inhibition of SPCA-1 Cell Proliferation byExtracts of Hericium erinaceus Fruit Bodies |
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| Abstract: | Dried Hericium erinaceus fruit bodies(500 g)were ground to a powder, extracted four times with 5 L of 95% ethanol for 24 h at room temperature (~20 ℃), and the combined supernatants were evaporated to dryness under vacuum at 55 ℃.After dissolving the residue in 200 mL ddH2O, the solution was extracted three times each with 200 mL petroleum ether, chloroform, ethyl acetate and n-butyl alcohol, in sequence.In each case, the combined extracts were again evaporated to dryness under vacuum and 40 mg of the residue material obtained taken up in 1 mL DMSO.Material extracted with petroleum ether inhibited the proliferation of SPCA-1 lung cancer cells in vitro, stimulated the release of reactive oxygen species, induced early cell apoptosis, and curtailed the duration of the G0/G1cell cycle phases.None of these effects were recorded using material extracted using the other solvents. |
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| Keywords: | Petroleum ether extract inhibition of tissue cell proliferation apoptosis cell cycle |
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