| 猴头菌子实体醇提物的生物活性 |
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| 引用本文： | 冯娜,韩乐,颜梦秋,马迪,韩伟,张劲松,唐庆九.猴头菌子实体醇提物的生物活性[J].食用菌学报,2017(1):67-71. |
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| 作者姓名： | 冯娜 韩乐 颜梦秋 马迪 韩伟 张劲松 唐庆九 |
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| 作者单位： | 1. 上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海 201403;2. 华东理工大学药学院,上海,200237 |
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| 基金项目： | 上海市农委(攻关基础)[沪农科攻字(2016)第6-3-3号]资助 |
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| 摘 要： | 通过对猴头菌(Hericium erinaceus)子实体的乙醇提取物进行萃取,得到了石油醚、氯仿、乙酸乙酯、正丁醇和剩余水分部共5个分部,通过定性鉴定分析了其中次生代谢物质的成分,并以保护PC12细胞损伤和抑制DPP-IV酶的活性为实验模型,寻找具有抗衰老、降血糖等生物活性组分。结果表明,猴头菌子实体的醇提取物中可能含有生物碱、酚类(或鞣质)、甾类(或萜类)、蒽醌等物质。5个分部对NaN3诱导所致的PC12细胞损伤均具有一定恢复作用,与对照(69.5%)比,剩余水分部活性最强(84.6%)。石油醚分部和剩余分部在浓度为1.0mg/mL时对DPP-IV酶的抑制率超过了阳性对照药(25.25%),而氯仿萃取分部在浓度为0.4mg/mL时活性已超过阳性对照药,说明氯仿分部具有较强的降血糖潜力。
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| 关 键 词： | 猴头菌 保护PC12细胞损伤 DPP-IV酶抑制活性 |
Major Chemical Components and Biological Activityof Different Fractions from Ethanolic Extractof Hericium eriaceus Fruit Bodies |
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| Abstract: | Five fractions were separated through different organic solvents (petroleum ether, CHCl3, EtOAc, n-BuOH and residue) from 95% ethanol extract of Hericium erinaceus fruit bodies.All the fractions exhibited a capacity for repair damage to PC12 cells induced by NaN3,and inhibitory activity on DPP-IV enzyme(0.2~1.0 mg/mL).Composition was varied in these fractions such as alkaloid, sugars, amino acids and protein, organic acids, phendic, sterides, anthraquinone, but no flavonoids was detected. |
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| Keywords: | Hericium erinaceus protection PC12 cell injury DPP-IV enzyme inhibitory activity |
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