巴氏蘑菇多糖对糖尿病小鼠脂代谢紊乱的干预作用

选择健康雄性昆明小鼠随机分组,建立糖尿病小鼠模型后分别灌胃不同剂量的巴氏蘑菇(Agaricus blazei)多糖(低、中和高剂量组分别灌胃50、100和200mg/kg/d),阳性对照组灌胃200mg/kg/d阿卡波糖,正常对照和模型组灌胃生理盐水,7周后考察巴氏蘑菇多糖对糖尿病小鼠脂代谢的影响。结果表明:与糖尿病模型组相比,多糖各剂量组和阳性对照组空腹血糖值和血清总胆固醇含量均显著下降;多糖中、高剂量组和阳性对照组的血清甘油三酯含量也显著下降;与模型组相比,附睾脂肪中阳性对照组的glut4、pi3k、akt1和akt2mRNA的表达量均显著升高,多糖组中glut4(低、中和高剂量组)、akt1(低、中和高剂量组)、akt2(中和高剂量组)和pi3k(高剂量组)mRNA的表达量显著升高。

Effects of Agaricus blazei Polysaccharide onLipid-associated Metabolic Factors in Diabetic Mice

The effects of polysaccharide material extracted from Agaricus blazei fruit bodies on lipid-associated metabolic factors in diabetic mice are described.Forty-eight healthy (30±2) g male Kunming mice were randomly divided equally into six groups as follows: normal control (NC), diabetic model (Model), low, medium and high doses of A.blazei polysaccharide (L, M and H, respectively), and positive control (PC).Diabetic mice were prepared by injection with 2% alloxan (200 mg/kg/d body weight) for three days.Mice in L, M and H groups were administered 50, 100 and 200 mg/kg/d polysaccharide intragastrically respectively, while PC mice received 200 mg/kg/d acarbose intragastrically.Mice in both the NC and Model groups were administered physiological saline intragastrically.After 7 weeks, blood glucose levels, total cholesterol (TC) and triglyceride (TG) levels in serum, and levels of glut4, pi3k, akt1 and akt2 expression in epididymal fat were measured.Blood glucose and TC levels in all three polysaccharide-treated and PC mice were significantly lower compared with mice in the Model group.TG levels in M, H and PC mice were also significantly lower.Expression levels of glut4 and akt1 mRNAs were significantly higher in L, M and H mice, akt2 mRNA expression was significantly higher in M and H groups, and pi3k mRNA expression was significantly higher in H group mice, compared with mice in the Model group.Mice in the PC group all exhibited significantly higher levels of glut4, pi3k, akt1 and akt2 mRNA expression.