八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响 |
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引用本文: | 于荣利,贾薇,张劲松,杨焱,吴迪,唐传红,颜梦秋,庄海宁,汪雯翰.八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响[J].食用菌学报,2017(2):88-92. |
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作者姓名: | 于荣利 贾薇 张劲松 杨焱 吴迪 唐传红 颜梦秋 庄海宁 汪雯翰 |
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作者单位: | 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心, 国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403 |
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基金项目: | 上海市农业科学院青年人才成长计划[沪农青字(2016)第1-14号]和上海市农业科学院科技发展基金[农科发2012(08)]资助 |
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摘 要: | 以乳腺癌细胞株MCF-7为实验对象,采用细胞增殖抑制率、细胞粘附抑制率、细胞克隆数和细胞迁移距离为评价指标,研究8种食药用菌醇提物对乳腺癌的影响。刺芹侧耳(Pleurotus eryngii)、姬松茸(Agaricus blazei)和灰树花(Grifola frondosa)的醇提物(200μg/mL)处理能显著降低MCF-7细胞的克隆形成数和细胞迁移距离,较阴性对照分别降低了64.3%、64.5%、61.6%和54.8%、54.8%、48.0%,3种醇提物对MCF-7细胞粘附数和细胞增殖的抑制率分别为48.5%、55.1%、49.3%和30.0%、34.1%、29.5%;另外5种食用菌醇提物作用效果不明显。结果初步表明,刺芹侧耳、姬松茸和灰树花的醇提物(200μg/mL)具有一定的体外抑制乳腺癌活性。
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关 键 词: | MCF-7细胞 细胞增殖 粘附能力 细胞迁移 细胞克隆 |
Effect of Ethanolic Extracts from Eight Kinds of Medicinal and Edible Fungi on Breast Cancer Cell MCF-7 in vitro |
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Abstract: | Effect of ethanolic extracts from medicinal and edible fungi (eight kinds) was evaluated on the breast cancer cell line, MCF-7, by detecting number of cell colon, inhibition rate of cell adherence, cell migration distance and inhibition rate of cell proliferationcell.Experimental data showed that pretreatments with 200 μg/mL Pleurotus eryngii, Agaricus blazei and Grifola frondosa extracts could significantly reduce clonal formation by 64.3%, 64.5%, 61.6% and cell migration distance by 54.8%,54.8%,48.0%, respectively, compared with negative control.Their inhibitory effects on the adhesion and cell proliferation were at the rate of 48.5%,55.1%,49.3% and 30.0%,34.1%,29.5%, respectively.But no significant effect on the four parameters of cells was recorded for other five tested extracts compared with negative control.Our data revealed that among 8 kinds of fungi evaluated in this study, only P.citrinopileus, A.blazei and G.frondosa showed anti-breast cancer activity in vitro. |
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Keywords: | MCF-7 cells cell proliferation adhesion cell migration cell cloning |
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