八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响

以乳腺癌细胞株MCF-7为实验对象,采用细胞增殖抑制率、细胞粘附抑制率、细胞克隆数和细胞迁移距离为评价指标,研究8种食药用菌醇提物对乳腺癌的影响。刺芹侧耳(Pleurotus eryngii)、姬松茸(Agaricus blazei)和灰树花(Grifola frondosa)的醇提物(200μg/mL)处理能显著降低MCF-7细胞的克隆形成数和细胞迁移距离,较阴性对照分别降低了64.3%、64.5%、61.6%和54.8%、54.8%、48.0%,3种醇提物对MCF-7细胞粘附数和细胞增殖的抑制率分别为48.5%、55.1%、49.3%和30.0%、34.1%、29.5%;另外5种食用菌醇提物作用效果不明显。结果初步表明,刺芹侧耳、姬松茸和灰树花的醇提物(200μg/mL)具有一定的体外抑制乳腺癌活性。

Effect of Ethanolic Extracts from Eight Kinds of Medicinal and Edible Fungi on Breast Cancer Cell MCF-7 in vitro

Effect of ethanolic extracts from medicinal and edible fungi (eight kinds) was evaluated on the breast cancer cell line, MCF-7, by detecting number of cell colon, inhibition rate of cell adherence, cell migration distance and inhibition rate of cell proliferationcell.Experimental data showed that pretreatments with 200 μg/mL Pleurotus eryngii, Agaricus blazei and Grifola frondosa extracts could significantly reduce clonal formation by 64.3%, 64.5%, 61.6% and cell migration distance by 54.8%,54.8%,48.0%, respectively, compared with negative control.Their inhibitory effects on the adhesion and cell proliferation were at the rate of 48.5%,55.1%,49.3% and 30.0%,34.1%,29.5%, respectively.But no significant effect on the four parameters of cells was recorded for other five tested extracts compared with negative control.Our data revealed that among 8 kinds of fungi evaluated in this study, only P.citrinopileus, A.blazei and G.frondosa showed anti-breast cancer activity in vitro.