DNA analysis of potato + Solanum brevidens somatic hybrid lines |
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| Authors: | Zsolt Polgar Susan M Wielgus Sandor Horvath John P Helgeson |
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| Institution: | (1) Department for Potato Research, Pannon University of Agricultural Sciences, Georgikon Faculty, 8360, Keszthely, Pf 71, Hungary;(2) Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, University of Wisconsin, Madison, WI, 53706, U.S.A |
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| Abstract: | Three somatic hybrid lines between potato (cv. While Lady line no. Ke 79, 2n = 2x = 48) + Solanum brevidens (PI 218228, 2n = 2x = 24) were evaluated using randomly amplified polymorphic DNA (RAPD) markers. The lines originated from the same callus but showed different reactions to Erwinia carotovora ssp. carotovora, the cause of potato soft rot. By the use of 48 oligomer primers producing 99 scorable bands, DNA polymorphism were detected on 7 of 12 S. brevidens chromosomes. Loss of certain DNA segments on chromosome 5, 6, 9 and 11 were observed. Some of the variations could have taken place in early callus stage of development; others may have occurred after initiation of individual shoot regeneration. The possible involvement of missing RAPD products specific to one somatic hybrid that shows decreased resistance to bacterial soft rot is discussed. |
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| Keywords: | Erwinia carotovora Solanum tuberosum somaclonal variation RAPD |
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