| Abstract: | After electrophoretic separation in SDS-PAGE structural proteins of the virus of Equine Infectious Anemia (EIA) were easily blotted by the semi-dry-blotting method onto nitrocellulose filters. Strips of these filters were used for antibody demonstration, and positive reactions thereof were intensified by a biotin-avidin-peroxidase system. Sensitivity of this system was so high as to allow readable interpretation of bands up to the dilution of 1:6,400 of a strongly positive serum. Frequently this procedure allowed to make a firm diagnostic Western-Blot diagnosis on far weaker equine sera. Interpretation of results proved, however, difficult with sera, which formed one single band only. This observation, although of weak grade, had to be made in some 5% of sera stemming from horses with a certainly negative history of EIA. Consequently, we conclude, that a policy followed in the serodiagnostic Western-Blot of human AIDS should also be adopted for the interpretation of the EIA Western-Blot, namely to declare as positive merely horse sera which evidence more than one single band, whereof at least one band should represent a viral glycoprotein. |
