固态热带假丝酵母菌菌剂的发酵工艺研究

试验在单因素优化试验的基础上,采用正交试验进一步对热带假丝酵母菌固态发酵工艺进行研究,包括发酵时间、接种量、料水比、碳源、氮源、无机盐等因素,最终确定热带假丝酵母菌的固态发酵工艺。发酵条件:在自然pH值条件下,发酵温度30℃、发酵时间48 h、接种量11%、料水比1∶0.75。培养基成分:麸皮与甘油8.5∶1.5 (g/g)、尿素0.5%、磷酸二氢钾0。依照此工艺进行发酵,热带假丝酵母菌活菌数最高可达32.94×10⁸个/g。

Study on solid state fermentation process of Candida tropicalis

Based on the optimization experiment of single factor, the further study of the solid fermentation process of Candida tropicalis was carried out by orthogonal experiment. Including fermentation time, inoculation amount, ratio of material to water, carbon source, nitrogen source, inorganic salt and other factors. The fermentation conditions were natural pH value, fermentation temperature of 30 ℃, fermentation time of 48 h, inoculation amount of 11%, the ratio of material to water of 1∶0.75. Medium compositions were bran and glycerol(g) 8.5∶1.5, urea 0.5%, potassium dihydrogen phosphate 0. In the process for fermentation, the number of viable count Candida tropicalis up to 32.94×10⁸/g.