黄淮山羊H FABP基因多态性及其与肌内脂肪含量的关联分析

 运用PCR SSCP技术检测黄淮山羊H FABP基因部分外显子和内含子区域的多态性，采用最小二乘均数法对其与IMF含量的关系进行分析。在2对扩增引物中只检测到1个多态位点，表现为GG和GC两种基因型。GC基因型个体的IMF含量显著高于GG基因型个体，在背最长肌和腿肌中GC基因型个体的IMF含量显著高于GG基因型（P<005）；在胸肌中，GG基因型与GC基因型个体之间的IMF含量无显著差异（P>005）。据此推测，H-FABP基因该多态位点的GC基因型可能是IMF含量性状的有效标记基因型。

Polymorphism and Association with IMF Content of H FABP Gene in Huanghuai Goat

The polymorphism of partial exon and intron of H FABP gene was detected by PCR SSCP. There were only one polymorphism site occurring in two primer amplifications, where showed two genotypes GG and GC. The association between the polymorphism and IMF content was analyzed and the results revealed that the IMF content of the longissimus dorsi muscles and leg muscles from GC genotype individuals was significantly higher than that of GG genotype individuals (P<005). Results also demonstrated no significant difference had been detected in the IMF content of the breast muscles between GG genotype and GC genotype （P>005）. Our experiment suggested that the H FABP gene could be a useful candidate gene in increasing the IMF content of goats.