猪型布鲁氏菌Omp31基因的抗原表位预测与分析

 布鲁氏菌的外膜蛋白在维持细胞结构方面发挥着重要作用，其中，有些外膜蛋白与细菌本身的毒性相关，成为了重要的抗原决定簇相关分子。Omp31分子就是猪型布鲁氏菌的一个重要的抗原表位分子，在研制亚单位疫苗方面有重要作用。本论文以猪型布鲁氏菌Omp31基因作为研究对象，利用DNAStar生物学软件对Omp31分子的立体结构、分子的表面可能性、亲水性、蛋白骨架区的柔韧性、分子的抗原指数进行了详细分析。结果显示：猪型布鲁氏菌Omp31分子一级结构的氨基酸序列中存在4段可能的抗原表位区段，其中，51~80氨基酸残基组成的区段和193~228氨基酸残基组成的区段的抗原指数较高，成为抗原决定簇的可能性很大。

Antigen Epitope Prediction and Analysis of Swine Brucella Omp31 Gene

Brucella outer membrane proteins play an important part in maintaining cell structure. Some outer membrane proteins associated with bacterial toxicity. And they become important antigenic determinants related molecules. Swine Brucella Omp31 is an important antigen epitope molecule, and plays an important part in researching and making subunit vaccine. In this study, swine Brucella Omp31 was research object. And the molecular structure, hydrophilic, protein skeleton zone flexibility, molecular antigen index were analyzed, using DNAStar biology software. Results showed that the amino acids sequence of swine Brucella Omp31 molecular included four possible epitopes section. And the antigen index of 51~80 amino acids segment and 193~228 amino acids segment was larger. The possibility of becoming antigenic determinants was much greater.