| 烟草疫霉的快速分子检测 |
| |
| 引用本文： | 高乔芬,秦西云,方敦煌,莫笑晗,杨根华,陈海如,蔡红.烟草疫霉的快速分子检测[J].云南农业大学学报,2012,27(2):156-160. |
| |
| 作者姓名： | 高乔芬 秦西云 方敦煌 莫笑晗 杨根华 陈海如 蔡红 |
| |
| 作者单位： | 1. 云南农业大学 植物保护学院，农业生物多样性与病虫害控制教育部重点实验室，云南 昆明 650201；2.云南省烟草农业科学研究院，云南 玉溪 653100 |
| |
| 摘 要： | 根据烟草疫霉与其他疫霉菌ITS序列的差异设计了1对寡聚核苷酸引物，用于烟草疫霉的PCR检测。结果表明，在优化的反应体系与扩增条件下，该对引物表现出强特异性，只在烟草疫霉为模板的扩增体系中具有1条660bp的条带。利用此特异性引物可稳定地从含有烟草疫霉的土壤及其发病组织中检测出病原菌。本实验提供并完善了一套快速检测烟草疫霉的方法和技术，特别对土传病害的准确诊断与快速检测在实践和理论上具有重要意义。
|
| 关 键 词： | 烟草疫霉 特异引物 分子检测 |
A Rapid Method to Detect Phytophthora nicotianae by PCR |
| |
| GAO Qiao-fen,QIN Xi-yun,FANG Dun-huang,MO Xiao-han,YANG Gen-hua,CHEN Hai-ru,CAI Hong.A Rapid Method to Detect Phytophthora nicotianae by PCR[J].Journal of Yunnan Agricultural University,2012,27(2):156-160. |
| |
| Authors: | GAO Qiao-fen QIN Xi-yun FANG Dun-huang MO Xiao-han YANG Gen-hua CHEN Hai-ru CAI Hong |
| |
| Institution: | 1. Key Laboratory for Agricultural Biodiversity and Pest Management of China Education Ministry, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China ; 2.Yunnan Tobacco Science Research Institute, Yuxi 653100, China |
| |
| Abstract: | According to the sequence of internal transcribed spacer regions (ITS) of the ribosomal gene, a pair of specific primers for Phytophthora nicotianae was synthesized. In the optimization of reaction conditions and amplification, only a single band of PCR product about 660 bp was amplified from P. nicotianae in soil or the diseased tobacco tissues. In this study, we provided a rapid method to detect P. nicotianae. It has great significance for accurate diagnosis and rapid detection of the soil borne pathogen. |
| |
| Keywords: | Phytophthora nicotianae specific primer molecular detection |
|
| 点击此处可从《云南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《云南农业大学学报》下载免费的PDF全文 |
