| 奶牛裸露冷冻半胚移植试验 |
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| 引用本文： | 柏学进,李洪祥.奶牛裸露冷冻半胚移植试验[J].中国兽医学报,1997,17(2):109-111. |
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| 作者姓名： | 柏学进 李洪祥 |
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| 作者单位： | 黑龙江省畜牧研究所，齐齐哈尔市新中畜牧场 |
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| 摘 要： | 对以１０％甘油＋１０％葡聚糖（Ｔ－５００）＋０．１ｍｏｌ／Ｌ蔗糖／２０％ＦＣＳ－ＰＢＳ液裸露冷冻的奶牛半胚进行了移植试验。移植妊娠率为３９．４％（２８／７１），并获得移植犊牛。试验结果表明，移植单枚或１对半胚的妊娠率无显著差异；在受体牛发情后８～１２ｈ肌注ＬＲＨ－Ａ３４００μｇ，获得５０％的移植妊娠率，极显著地高于对照组（３８．５％～４０％）。此外，移植妊娠率具有随着胚胎发育阶段升高而增高的趋势。
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| 关 键 词： | 奶牛 冷冻半胚 移植 |
Transfer of Zona free Frozen demi embryos in Milk Cattle |
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| BaiXuejin,Wu Tierong,Xue Jianhua,Yue Kui zhong,Dong YaJuan,Zhang Xinhui,Luo Mingjiu,Huang Zhongmu,Zhong Shifang.Transfer of Zona free Frozen demi embryos in Milk Cattle[J].Chinese Journal of Veterinary Science,1997,17(2):109-111. |
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| Authors: | BaiXuejin Wu Tierong Xue Jianhua Yue Kui zhong Dong YaJuan Zhang Xinhui Luo Mingjiu Huang Zhongmu Zhong Shifang |
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| Abstract: | Milk cattle demi embryos of zona free cryopreservation with10%Glycerol 10?xtran(T 500) 0.1mol/LSucrose/20?S PBS were transferred to synchronous recipients.Transferred calves had obtained with the pregnancy rate to be39.4%(28/71).The results showed that pregnancy rate did not differ( P >0.05)with single demi embryos or double demi embryos.The transferred pregnancy rate(50%)obtained in recipients injected with LRH A 3at8 12hours after es trus was higher significantly(<0.01)than that of control(38.5% 40%).The transferred preg nancy rate seems to be progress with the development stage of embryos. |
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| Keywords: | milk cattle cryopreservation demi embryos transfer |
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