| PCR扩增ZFY,ZFX序列鉴定奶牛性别的灵敏度研究 |
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| 引用本文： | 龚荣慈 唐榕. PCR扩增ZFY,ZFX序列鉴定奶牛性别的灵敏度研究[J]. 中国兽医学报, 1997, 17(3): 234-237 |
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| 作者姓名： | 龚荣慈 唐榕 |
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| 作者单位： | 西南民族学院牧医系，复旦大学遗传研究所 |
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| 基金项目： | 复旦大学国家基因工程重点实验室项目,国家民委“八五”重点项目 |
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| 摘 要： | 从克隆、测序所得奶牛ＺＦＹ、ＺＦＸ序列中设计１对高特异性引物（或探针），即分别特异于牛ＺＦＹ、ＺＦＸ的ＺＦ３、ＺＦ４，与克隆时使用的引物ＺＦ２搭配成ＺＦ２、ＺＦ３和ＺＦ２、ＺＦ４。利用这２对引物和ＺＦ１、ＺＦ２对奶牛基因组ＤＮＡ进行ＮｅｓｔＰＣＲ来判断性别，结果达到用超微量（８ｐｇ）ＤＮＡ，即可检出ＺＦＹ、ＺＦＸ序列而判定雌雄的灵敏度；用非同位素标记试剂盒（ＤＩＧ－Ｓｙｓｔｅｍ）标记ＺＦ３、ＺＦ４作为探针，对ＺＦ１、ＺＦ２扩增产物点杂交来判断性别，也达到同样的灵敏度
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| 关 键 词： | 牛 性别鉴定 PCR ZFY ZFX |
Sensibility of Sex Identification with Amplification of ZFY and ZFX Gene Sequences in Bovine by PCR |
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| Gong Rongci Xu Yaou. Sensibility of Sex Identification with Amplification of ZFY and ZFX Gene Sequences in Bovine by PCR[J]. Chinese Journal of Veterinary Science, 1997, 17(3): 234-237 |
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| Authors: | Gong Rongci Xu Yaou |
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| Abstract: | The sequences of ZFY and ZFX gene fragments obtained from cloning and sequencing in bovine were analyzed. It was found that there were 88.7% homologous region within the two fragments. The remains appeared to be different. According to these different sequences, the primers or probe (ASO) ZF 3 specific for ZFY and the ZF 4 specific for ZFX were designed. One set of ZFY specific primers formed by ZF 2 and ZF 3 and the another set of ZFX specific primers formed by ZF 2 and ZF 4 were used to identify the sex of bovine by nest PCR. The results showed that a 8 pg DNA level of the sensibility for sex identification achieved in bovine by the nest PCR. |
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| Keywords: | bovine sex identification PCR ZFY ZFX |
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