| 乳白石蒜17份种质资源的RAPD分析 |
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| 引用本文： | 刘志高,童再康,黄华宏,高燕会.乳白石蒜17份种质资源的RAPD分析[J].西北林学院学报,2012,27(4):113-116,123. |
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| 作者姓名： | 刘志高 童再康 黄华宏 高燕会 |
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| 作者单位： | 1. 浙江农林大学园林学院,浙江临安311300;
2. 浙江农林大学林业与生物技术学院,浙江临安,311300 |
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| 基金项目： | 浙江省重点科技创新团队子项目(2011R50034-03);浙江省教育厅项目(Y200907683);校发基金:离子注入对石蒜属植物花、叶变异的影响研究(2010FK048) |
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| 摘 要： | 为分析不同种源乳白石蒜材料的亲缘关系,利用RAPD标记对乳白石蒜的17份样品的遗传多样性进行了分析。结果表明:从200个随机引物中筛选出33个多态性较高的引物,扩增出623条DNA带,其中527条为多态带,占84.6%,平均每个引物扩增的DNA带数为18.88条。根据RAPD扩增结果,将乳白石蒜各样品进行聚类,在阈值为0.352时划分为3类。分子聚类结果与原产地和花形态特征具有一定的联系。
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| 关 键 词： | 乳白石蒜 RAPD 遗传多样性 |
RAPD Analysis on 17 Accessions of Lycoris albiflora Germplasm Resources |
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| LIU Zhi-gao
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TONG Zai-kang
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HUANG Hua-hong
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GAO Yan-hui.RAPD Analysis on 17 Accessions of Lycoris albiflora Germplasm Resources[J].Journal of Northwest Forestry University,2012,27(4):113-116,123. |
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| Authors: | LIU Zhi-gao TONG Zai-kang HUANG Hua-hong GAO Yan-hui |
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| Institution: | 1.College of Landscape Architecture,Zhejiang A&F University,Lin’an,Zhejiang 311300,China; 2.College of Forestry and Life Sciences,Zhejiang A&F University,Lin’an,Zhejiang 311300,China) |
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| Abstract: | In order to verify the genetic relationship of Lycoris albiflora,the genetic diversity of 17 different varieties of L.albiflora were analyzed by using the random amplified polymorphic DNA(RAPD) in the study.The results were as follows: 33 effective primers were selected from 200 primers,which generated 623 bands in total,and 527 bands showed polymorphism,the average polymorphism rate was 84.6%.The dendrograms of 17 different variations were generated by UPGMA method,and were divided into 3 groups when genetic distance coefficient was given as 0.352.The results of molecular dendrogram demonstrated some connections with phylogenetic and origin of 17 different variations of L.albiflora. |
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| Keywords: | Lycoris albiflora RAPD genetic diversity |
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