| 牡丹幼嫩花瓣愈伤组织诱导及芽的分化 |
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| 引用本文： | 朱向涛,王雁,彭镇华,律春燕.牡丹幼嫩花瓣愈伤组织诱导及芽的分化[J].西北林学院学报,2012(1):85-87,264. |
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| 作者姓名： | 朱向涛 王雁 彭镇华 律春燕 |
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| 作者单位： | 浙江农林大学天目学院;中国林业科学研究院林业研究所国家林业局林木培育重点试验室;胶州市少海发展管理处 |
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| 基金项目： | 科技部863项目(2007AA10Z182);国家林业局‘948’项目(2006-4-C07) |
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| 摘 要： | 以牡丹"凤丹"的花瓣为外植体,在优化植物生长调节剂的基础上,成功诱导出愈伤组织并实现植株再生,对愈伤组织诱导和分化的培养基类型、植物生长调节剂种类和浓度进行筛选,结果表明:不同的生长调节剂对愈伤组织诱导和分化的影响不同,愈伤组织诱导的最佳培养基为MS+2,4-D 2.0mg.L-1+6-BA 1.5mg.L-1+NAA 0.3mg.L-1,愈伤组织分化的最佳培养基为:MS+ZT 0.5mg.L-1+6-BA 2.0mg.L-1,分化率达到59.0%。
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| 关 键 词： | 牡丹花瓣 愈伤组织 诱导 分化 |
Callus Induction and Bud Differentiation of Paeonia suffruticosa Petal |
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| ZHU Xiang-tao,WANG Yan,PENG Zhen-hua,LV Chun-yan.Callus Induction and Bud Differentiation of Paeonia suffruticosa Petal[J].Journal of Northwest Forestry University,2012(1):85-87,264. |
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| Authors: | ZHU Xiang-tao WANG Yan PENG Zhen-hua LV Chun-yan |
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| Institution: | 2,3(1.Tianmu college,Zhejiang Agricultural and Forestry University,Linan Zhejiang 311300;2.Research Institute of Forestry, Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091; 3.Jiaozhou Shaohai Development Management Department,Qingdao,Shandong 266300) |
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| Abstract: | The petals of Paeonia suffruticosa were used as explants in this experiment to examine the roles of plant hormones combinations for callus formation and shoot differentiation.The results showed that plant hormones exhibited different impacts on the callus induclion and shoot differentiation.The best plant hormone combinations for callus induction were MS+2,4-D 2.0 mg·L-1+6-BA 1.5 mg·L-1+NAA 0.3 mg·L-1 and MS+ZT 0.5 mg·L-1+6-BA 2.0 mg·L-1 was the best plant combination for callus differentiation,the rate of callus differentiation was 59.0%. |
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| Keywords: | Paeonia suffruticosa petal callus induction differentiation |
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