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| 毛果杨全基因组硝酸根转运蛋白家族(NRT2s)序列分析 | |
| 引用本文： | 袁丁,李红,郝文芳,罗志斌.毛果杨全基因组硝酸根转运蛋白家族(NRT2s)序列分析[J].西北林学院学报,2012,27(5):80-86. |
| 作者姓名： | 袁丁 李红 郝文芳 罗志斌 |
| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西杨陵,712100 2. 西北农林科技大学植物保护学院,陕西杨陵,712100 3. 西北农林科技大学生命科学学院,陕西杨陵712100;西北农林科技大学林学院,陕西杨陵712100 |
| 基金项目： | 国家重点基础研究发展计划项目(2012CB416902);教育部新世纪优秀人才支持计划项目(NCET-08-0468);教育部高等学校博士学科点专项科研基金课题(20090204110027);霍英东教育基金会课题(121026) |
| 摘 要： | 以拟南芥(Arabidopsis thaliana)和莱茵衣藻(Chlamydomonas reinhardttii)高亲和性硝酸根转运蛋白(NRT2s)为对照,对毛果杨(Populus trichocarpa)NRT2s家族成员进行序列比对、系统发育分析、基因结构分析、理化性质预测、疏水性与跨膜区预测、结构域检测等生物信息学分析。结果表明:毛果杨基因组包含6个NRT2s家族成员并可分为3个亚家族。其中PtNRT2.1、PtNRT2.2和PtNRT2.3属于亚家族I;PtNRT2.4和PtNRT2.5属于亚家族II;PtNRT2.6属于亚家族III。亚家族I保守程度最高,亚家族II和III可能由亚家族I进化而来。所有的NRT2s都具有高度相似的结构域、功能区和基因结构,在不同物种中的进化较为保守。不同的NRT2s成员定位于不同的亚细胞结构中,其具体功能可能存在差异,并共同参与调控杨树的硝态氮平衡。 |
| 关 键 词： | 氮素营养 硝酸根 转运蛋白 毛果杨 序列分析 |
Genome-wide Sequence Analysis of High Affinity Nitrate Transporter(NRT2s)Gene Family in Populus trichocarpa |
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| YUAN Ding , LI Hong , HAO Wen-fang , LUO Zhi-bin.Genome-wide Sequence Analysis of High Affinity Nitrate Transporter(NRT2s)Gene Family in Populus trichocarpa[J].Journal of Northwest Forestry University,2012,27(5):80-86. | |
| Authors: | YUAN Ding LI Hong HAO Wen-fang LUO Zhi-bin |
| Institution: | 1,3(1.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi,712100,China;2.College of Plant Protection, Northwest A&F University,Yangling,Shaanxi,712100,China;3.College of Forestry, Northwest A&F University,Yangling,Shaanxi,712100,China) |
| Abstract: | Based on gene sequences of putative high affinity nitrate transporters(NRT2s) in Populus trichocarpa,gene structures,physicochemical parameters,hydrophobicity,subcellular localization,transmembrane regions and conserved domains were predicted with bioinformatics methods using NRT2s in Arabidopsis thaliana(AtNRT2s) and Chlamydomonas reinhardttii(CreNRT2s) as comparison.In P.trichocarpa there were 6 NRT2s family members named as PtNRT2.1,PtNRT2.2,PtNRT2.3,PtNRT2.4,PtNRT2.5 and PtNRT2.6,which could be divided into 3 subfamilies according to their highly conserved sequences and functional domains.PtNRT2.1,PtNRT2.2,and PtNRT2.3 could be in subfamily I,while PtNRT2.4,PtNRT2.5 in subfamily II and PtNRT2.6 in subfamily III.Although PtNRT2s had similar amino acid sequences,they showed variations in subcellular localization.These data indicated that PtNRT2s probably play different roles in nitrate balance in P.trichocarpa. |
| Keywords: | Nitrogen nutrition nitrate transporter Populus trichocarpa sequence analysis |
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