均匀设计和回归法优化苦楝素提取工艺研究

运用均匀设计和回归分析方法优化植物源农药苦楝素的提取工艺。采用溶剂浸提法从苦楝果实中提取苦楝素,考察提取工艺中石油醚去酯、乙醇回流提取(95℃)和乙酸乙酯萃取过程对苦楝素提取率的影响。经优化筛选,得到苦楝素最优提取工艺为:石油醚浸提的液料比1∶24(g∶mL),时间20min,无水乙醇回流提取的液料比1∶34(g∶mL),时间390min,125mL乙酸乙酯萃取100min,提取率达0.721 3%。

Extraction of Toosendanin with Uniform Design and Regression Analysis

Uniform design and regression analysis were adopted to optimize the extraction process of toosendanin,a kind of vegetal pesticide by using petroleum ether to remove lipids and ethanol to extract target constituents from the fruit of chinaberry.The optimal conditions were as follows.In the procedure of removing lipids,the ratio of petroleum ether to fruit material was 1∶24(g:mL) with a refluxing time of 20 min.In the extraction procedure,the ratio of absolute ethanol to the defatted fruit residue was 1∶34(g:mL) with the refluxing time of 390 min.After ethanol was removed,the extract was further extracted by ethyl acetate for 100 min,the yield of toosendanin reached 0.721 3%,indicating that uniform design and regression analysis were appropriate to determine the control parameters for the multi-factor and multi-level experiment.