华山松大小蠹成虫粪便挥发性物质分析

采用固相微萃取(SPME)和气相色谱-质谱联用(GC-MS)技术,对华山松大小蠹(Dendroc-tonus armandi Tsai et Li)成虫新鲜粪便和华山松韧皮部中的挥发性物质进行了分析。结果表明,华山松大小蠹雌雄成虫新鲜粪便和华山松韧皮部中挥发性物质以倍半萜类物质最多,其次是单萜类物质,而未见有二萜类物质,华山松大小蠹雌雄成虫粪便和华山松韧皮部中挥发性物质在成分和含量上都有较大差别,粪便中单萜类物质含量明显高于韧皮部,而倍半萜含量则低于韧皮部。此外,6,6-二甲基-2-甲醛基-双环3.1.1]庚-2-烯、1,3,3-三甲基-2-羟基-双环2.2.1]庚烷、2,3-二甲基-3-甲氧基-三环2.2.1.0(2,6)]庚烷等15种挥发性物质仅存在于华山松大小蠹成虫粪便中;4,6,6-三甲基-(1s)-双环3.1.1]庚-3-烯-2-酮、1,3,3-三甲基-2-酮-双环2.2.1]庚烷、对位烯丙基-苯甲醚等8种挥发性物质只存在于雌性成虫粪便中;3,7-二甲基-1,6-辛二烯-3-醇、2,2,3-三甲基-1-乙醛基-3-环戊烯和二叔丁基对甲酚仅存在于雄性成虫粪便中。为下一步研究华山松大小蠹成虫对寄主华山松的选择性和华山松大小蠹信息素提供一定的理论基础。

Volatile Compounds in the Frass of Adult Chinese White Pine Beetle

Volatile compounds from phloem of healthy Chinese white pine(Pinus armandi Franch) and fresh frass of Chinese white pine beetle(Dendroctonus armandi Tsai et Li) were extracted by solid-phase microextraction and analyzed by gas chromatography-mass spectrometry(GC-MS).The results show that the most substances were sesquitertenes,followed by monoterpenes,no diterpenes were detected in frass and phloem.There was significant difference both in constituents and contents between beetle adult frass and trees phloem.The content of monoterpene was much more but the sesquitertene was less in frass of Chinese white pine beetle than those in phloem of healthy Chinese white pine.Fifteen substances such as 6,6-dimethyl-bicyclo hept-2-ene-2-carboxaldehyde,1,3,3-trimethyl-bicycloheptan-2-ol,2,3-dimethyl-tricycle 2.2.1.0(2,6)] heptane-3-methanol,and so on were detected only in adult D.armandi frass,of which eight substances such as 4,6,6-trimethyl-(1S)-bicyclehept-3-en-2-one,1,3,3-trimethyl-bicycleheptan-2-one,pallyl-anisole were only detected in female frass and 3,7-dimethyl-1,6-octadien-3-ol,2,2,3-trimethyl-3-cyclopentene-1-acetaldehyde,butylated hydroxytoluene in male frass,which provided theoretical basis to the host selection of adults D.armandi to Chinese white pine and secretion of D.armandi pheromone.