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枣未成熟胚高效再生体系研究
引用本文:崔秀梅,王玖瑞,刘孟军,代丽,郭敬丽.枣未成熟胚高效再生体系研究[J].西北林学院学报,2012(1):81-84.
作者姓名:崔秀梅  王玖瑞  刘孟军  代丽  郭敬丽
作者单位:河北农业大学林学院;河北农业大学中国枣研究中心
基金项目:国家自然科学基金资助项目(30471194);河北农业大学博士科研补助资金项目
摘    要:以枣树雄性不育3号(JMS3)和金芒果枣为材料,建立了枣树未成熟胚高效再生植株体系。对枣未成熟胚高效再生体系研究结果表明:枣未成熟胚可以通过直接萌发和经子叶发生愈伤两条途径再生完整植株,冷藏处理是促进未成熟胚直接成苗的有效方法。枣未成熟胚经0~4℃冷藏60d,接种在MS+蔗糖30g.L-1+琼脂3.3g.L-1培养基上,胚直接成苗率高达93.3%。将去除核壳时受损伤胚的子叶接种到MS+麦芽糖20g.L-1+琼脂3.3g.L-1+TDZ 1.0mg.L-1+NAA 0.2mg.L-1培养基上,40d后出愈率为98.9%。所得愈伤经MS+麦芽糖20g.L-1+琼脂3.3g.L-1+TDZ 1.0mg.L-1诱导40d后出芽率可达74.1%,丛生芽率达70.4%。在生根培养基1/2MS+蔗糖20g.L-1+琼脂3.3g.L-1+IBA1.0mg.L-1+IAA0.01mg.L-1上40d后生根率为80.8%。

关 键 词:  未成熟胚  子叶  植株再生

An Efficient System for Plant Regeneration from Immature Embryo of Chinese Jujube
CUI Xiu-mei,WANG Jiu-rui,LIU Meng-jun,DAI Li,GUO Jing-li.An Efficient System for Plant Regeneration from Immature Embryo of Chinese Jujube[J].Journal of Northwest Forestry University,2012(1):81-84.
Authors:CUI Xiu-mei  WANG Jiu-rui  LIU Meng-jun  DAI Li  GUO Jing-li
Institution:1(1.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071001,China; 2.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding,Hebei 071001,China)
Abstract:An efficient system for plant regeneration from immature embryo of Chinese jujube was established by using two callivars of male sterile No.3(JMS3) and ’Jinmangguozao’.The results showed that the plant regeneration could be achieved by two types of procedures: direct embryo germination and generation of callus of cotyledon.Cold treatment could efficiently accelerate the direct plant regeneration from immature embryo.The plant regeneration rate of immature embryos,which were kept at 0~4 ℃ for 60 days and then inoculated in medium MS + sugar 30 g·L-1+ agar 3.3 g·L-1,was as high as 93.3%.Callus could be induced from 98.9% of cotyledons damaged during smashing stones in medium MS +maltose 20 g·L-1+agar 3.3 g·L-1+TDZ 1.0 mg·L-1+NAA 0.2 mg·L-1 within 40 days.Adventitious buds occurred in 74.1% of the callus cultured in medium MS + maltose 20 g·L-1 +agar 3.3 g·L-1 +TDZ 1.0 mg·L-1 for 40 days with cluster buds rate of 70.4%.1/2MS + sugar 20 g·L-1+ agar 3.3 g·L-1+ IBA 1.0 mg·L-1 + IAA 0.01 mg·L-1 was proper rooting medium and the rooting rate reached 80.8% in 40 days.
Keywords:Chinese jujube(Ziziphus jujuba)  immature embryo  cotyledon  plant regeneration
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