| 火棘抑菌内生真菌的筛选与拮抗植物病原菌作用研究 |
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| 引用本文： | 田小曼,陈阿敏.火棘抑菌内生真菌的筛选与拮抗植物病原菌作用研究[J].西北林学院学报,2012,27(5):117-120. |
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| 作者姓名： | 田小曼 陈阿敏 |
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| 作者单位： | 1. 杨凌职业技术学院,陕西杨陵,712100
2. 陕西马鞍桥生态矿业有限公司,陕西周至,710400 |
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| 摘 要： | 采用常规分离方法从火棘的茎、叶、果实中分离得到15株内生真菌,以小麦根腐病菌、小麦赤霉病菌、番茄早疫病菌等9种病原真菌作靶标菌,筛选出了一株具有较高抑菌活性的菌株-J4,采用形态学观察和ITS序列测定相结合的方法对J4进行鉴定。结果表明,J4菌株为子囊菌亚门间座壳属(Diaporthe)真菌。
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| 关 键 词： | 火棘 内生真菌 分子鉴定 rDNA序列 |
Screening of Pyracantha fortuneana Endophytic Fungus and Function Examination of Antagonism to Plant Pathogenic Fungi |
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| TIAN Xiao-man
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CHEN A-min.Screening of Pyracantha fortuneana Endophytic Fungus and Function Examination of Antagonism to Plant Pathogenic Fungi[J].Journal of Northwest Forestry University,2012,27(5):117-120. |
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| Authors: | TIAN Xiao-man CHEN A-min |
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| Institution: | 1.Yangling Vocation and Technical College,Yangling,Shaanxi 712100,China; 2.Shaanxi Ma’anqiao Ecological & Mine Industry Co.,Ltd,Zhouzhi,Shaanxi 710400,China) |
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| Abstract: | Fifteen endophytic fungi were screened from the stems,leaves,and fruits of Pyracantha fortuneana.The antifungal activities of the fungi to 9 plant pathogenic fungi(including Bipolaris sorokiniana,Gibberella zeae,and Alternaria solani and so on) were selected.One strain,J4 with high antifungal activity was screened and obtained.Morphological observations and ITS sequence of strain J4 were conducted.The results showed that the strain belonged to ascomycetes Diaporthe genus. |
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| Keywords: | Pyracantha fortuneana endophytic fungus molecular identification rDNA sequence |
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