| 迟钝爱德华氏菌鞭毛蛋白的提取与分析 |
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| 引用本文： | 张晓佩,龚晖,陈如敬,杨金先,林天龙. 迟钝爱德华氏菌鞭毛蛋白的提取与分析[J]. 福建农业学报, 2008, 23(1): 35-38 |
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| 作者姓名： | 张晓佩 龚晖 陈如敬 杨金先 林天龙 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108;福建省农业科学院生物技术研究所,福建,福州,350003
2. 福建省农业科学院生物技术研究所,福建,福州,350003 |
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| 基金项目： | 农业部科技专项 , 福建省自然科学基金 , 福建省科技厅科研项目 |
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| 摘 要： | 采用不同的方法提取迟钝爱德华氏菌鞭毛蛋白(Edwardsiella Tardaflagellin,ETF),选出合适的方法提取ETF,同时制备了ETF的鼠抗血清和迟钝爱德华氏菌菌株ETY全菌的鼠抗血清,通过ELISA法和Western blottig法分析了ETF的抗原性和免疫原性。试验结果表明,酸化高速离心法可获得高纯度分子量约为44 kDa的ETF,该蛋白可被爱德华氏菌的全菌鼠抗血清识别,同时由ETF制备的鼠抗血清除了能特异识别该蛋白之外,也可识别爱德华菌菌体裂解产物中44 kDa的蛋白,证实爱德华氏菌鞭毛蛋白具有抗原性和免疫原性,可作为亚单位疫苗的候选抗原。
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| 关 键 词： | 鞭毛蛋白 迟钝爱德华氏菌 提取 抗血清 |
| 文章编号： | 1008-0384(2008)01-0035-04 |
| 修稿时间： | 2007-09-17 |
Purification and analysis of Edwardsiella Tarda flagellin |
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| ZHANG Xiao-pei,GONG Hui,CHEN Ru-jing,YANG Jin-xian,LIN Tian-long. Purification and analysis of Edwardsiella Tarda flagellin[J]. Fujian Journal of Agricultural Sciences, 2008, 23(1): 35-38 |
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| Authors: | ZHANG Xiao-pei GONG Hui CHEN Ru-jing YANG Jin-xian LIN Tian-long |
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| Abstract: | Methods for isolating Edwardsiella Tarda flagellin(ETF) from E.Tarda were studied.One method was selected.Mouse anti-ETF serum and anti-ETY serum were prepared.The antigenicity and immunogenicity of ETF were analyzed by means of ELISA and Western-blotting.The results showed that the method,i.e.,acidification and high-speed centrifuge,could obtain the highly purified ETF,which had a molecular weight about 44kD.The protein was recognized by the mouse anti-ETY and anti-ETF serum.In addition,the mouse anti-ETF serum could recognize the 44kD protein separated from ETY.This proves that ETF possesses antigenicity and immunogenicity.And,therefore,ETF could conceivably be a candidate for the preparation of the subunit vaccine against edwardsiellosis in Japonica eel. |
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| Keywords: | Edwardsiella tarda flagellin preparation anti-serum |
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