野生一粒小麦糖原合成酶激酶基因TbGSK1的克隆与分析

根据普通小麦糖原合成酶激酶基因序列设计引物,以野生一粒小麦cDNA为模板,利用RT-PCR技术,扩增出野生一粒小麦糖原合成酶激酶基因的cDNA序列,克隆到T载体上并进行测序。结果显示,该基因全长1543bp,开放阅读框1068bp,编码一个由355个氨基酸组成的蛋白。Southern blot分析显示,其在野生一粒小麦基因组中为单拷贝基因。该基因与普通小麦、玉米、烟草、苜蓿、拟南芥等植物的糖原合成酶激酶基因序列同源性分别达到94.1%、91.3%、83.2%、81.5%、72.8%。

Cloning and Sequrce Analysis of Glycogen Synthase Kinase Gene TbGSK1 in Triticum boeoticum Boiss

The PCR primers were designed based on the sequence of glycogen synthase kinase from Triticum aestivum and were used to amplify the cDNA fragment of glycogen synthase kinase gene in Triticum boeoticum Boiss by RT-PCR technique.The amplified fragment was further cloned and sequenced.The result showed that the full-length of the gene was 1543bp,contained an ORF of 1068bp,and encoded 355 amino acid residues.Southern blot analysis revealed that TbGSK1 was a single-copy gene in Triticum aestivum genome.The identity of the deduced amino acid sequences with that of Triticum boeoticum Boiss,Triticum aestivum,Zea mays,Nicotiana tabacum,Medicago sativa and Arabidopsis thaliana was 94.1%,91.3%,83.2%,81.5%,72.8%,respectively.