| 朱红橘及其突变体牛肉红金橘的AFLP分析 |
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| 引用本文： | 彭志军,陈守一,蔡永强,李金强,郭文武. 朱红橘及其突变体牛肉红金橘的AFLP分析[J]. 西南农业学报, 2011, 24(1) |
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| 作者姓名： | 彭志军 陈守一 蔡永强 李金强 郭文武 |
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| 作者单位： | 1. 贵州省果树科学研究所,贵州,贵阳,550006
2. 华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070 |
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| 基金项目： | 863计划项目子课题，贵州省农业科学院专项 |
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| 摘 要： | 为弄清朱红橘红色突变体牛肉红金橘遗传的真实性,利用AFLP分子标记技术对朱红橘(Citrus reticulata Blanco)及其实生变异体牛肉红金橘进行了遗传鉴定.结果表明:从120对EcoRI/MseⅠ酶切引物中筛选出具有多态性的引物7对(MCO5E04,MCO4E01,MC04E02,MC04E03,MC04E04,MC04E06,MCO5E02);对朱红橘及其突变体牛肉红金橘进行AFLP扩增,共扩增出648条带,其中,72条具有多态性,多态性比例占11.11%;应用NTSYSpc 2.1软件统计分析,品种(系)间的遗传距离为0.0215853.牛肉红金橘与朱红橘在DNA水平上确实存在差异,牛肉红金橘为遗传上稳定的变异体,具有成为新的红肉品种的遗传基础,可作为新型材料加以保护和利用.
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| 关 键 词： | AFLP分析 牛肉红金橘 朱红橘 突变体 遗传距离 |
Identification of Zhuhongju and Its Variant Line by AFLP Analysis |
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| PENG Zhi-jun,CHEN Shou-yi,CAI Yong-qiang,LI Jin-qiang,GUO Wen-wu. Identification of Zhuhongju and Its Variant Line by AFLP Analysis[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(1) |
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| Authors: | PENG Zhi-jun CHEN Shou-yi CAI Yong-qiang LI Jin-qiang GUO Wen-wu |
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| Affiliation: | PENG Zhi-jun1,CHEN Shou-yi1,CAI Yong-qiang1,LI Jin-qiang1,GUO Wen-wu2(1.Guizhou Fruit Institute,Guizhou Guiyang 550006,China,2.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Hubei Wuhan 430070,China) |
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| Abstract: | AFLP technique was used to determine DNA polymorphism of Zhuhongju and its variant line Niurou Hongjinju(Citrus reticulata Blanco) to study the genetic diversity of Zhuhongju(Citrus reticulata Blanco) and its red variant line.The results showed that seven primer pairs(MCO5-E04,MCO4-E01,MC04-E02,MC04-E03,MC04-E04,MC04-E06,MCO5-E02) were selected out of 120 pairs of primer combinations and 648 polymorphism bands were amplified,72 of which were polymorphic.Ratio of polymorphic bands was 11.11 %.The genetic dis... |
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| Keywords: | AFLP analysis Niurouhongjinju Zhuhongju Mutant Genetic distance |
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