| 藏红花愈伤组织的诱导及植株再生 |
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| 引用本文： | 王寿芹,赵永钦,刘莉莎,郭仰东. 藏红花愈伤组织的诱导及植株再生[J]. 西南农业学报, 2011, 24(1) |
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| 作者姓名： | 王寿芹 赵永钦 刘莉莎 郭仰东 |
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| 作者单位： | 1. 元谋县农业局,云南,元谋,651300
2. 中国农业大学农学与生物技术学院,北京,100193 |
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| 基金项目： | 人力资源和社会保障部少数民族科技骨干特殊培训项目资助 |
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| 摘 要： | 以藏红花(Crocus sativus L.)当年新生小球茎为材料,在MS+0.5 mg/L BA+1.0 mg/L NAA+7%CM(椰乳)培养基上进行初代培养,再转入MS+5 mg/L BA+5 mg/L NAA中进行愈伤组织的诱导,黑暗条件下继代1-2次,51 d就可以获得高诱导串的愈伤组织,愈伤诱导率可达65%.将愈伤组织转入分化培养基MS+5 mg/L BA+5 mg/L NAA中分化丛生芽,丛生芽诱导率高达95%,将丛生芽分成单个植株,最终形成完整再生植株.
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| 关 键 词： | 藏红花(Crocus sativus L.) 愈伤组织 丛生芽 再生 |
Callus Induction and Plant Regeneration of Saffron |
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| WANG Shou-qin,ZHAO Yong-qin,LIU Li-sha,GUO Yang-dong. Callus Induction and Plant Regeneration of Saffron[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(1) |
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| Authors: | WANG Shou-qin ZHAO Yong-qin LIU Li-sha GUO Yang-dong |
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| Affiliation: | WANG Shou-qin1,ZHAO Yong-qin2,LIU Li-sha2,GUO Yang-dong2(1.Agricultural Bureau of Yuanmou,Yunnan Yuanmou 651300,China,2.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | The corms of saffron were used as explants to establish a regeneration protocol for Crocus sativus L.The corms incubated in MS+0.5 mg/L BA +1.0 mg/L NAA+7 %CM at first,then transferred to MS +5 mg/ L BA +5 mg/ L NAA for callus induction.Callus formation was observed after 51 days,culture and the callus induction rate was 65 %.After four weeks callus induction,the shoot regeneration rate(95 %) was obtained when 2 mg/L BA was combined with 1 mg/L NAA.The shoot buds were fragmented and transferred to root indu... |
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| Keywords: | Crocus sativus L. Callus Buds Regeneration |
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