甜瓜种质资源亲缘关系的RAPD标记分析

采用RAPD标记方法,对22份甜瓜材料进行了品种遗传多样性分析。结果显示,22个引物共扩增 出189条DNA条带,其中143条表现出多态性,占总带数的75．66％。聚类分析结果表明,22个甜瓜品种(系)中首 先可分为薄皮甜瓜与厚皮甜瓜两大类,彼此亲缘关系最远;厚皮甜瓜中网纹甜瓜与厚皮甜瓜的其他类型又被分开; 再次一级的分类为河套蜜、皇后和剩余的厚皮甜瓜。对照田间表型观察结果可见,聚类分析结果与传统分类结果相 近。

Genetic relationship analysis of melon by RAPD marker

Twenty-two melon varieties were used to analyze genetic diversity by RAPD marker. Results showed that 189 DNA bands were amplfied with 22 random primers,of which 143 were polymorphic. An average proportion of polymorphic loci was 75.66%. Twenty-two varieties and inbreds were classified into two sub-groups ,which were thick-peel melon and thin-peel melon. The cantaloupe was far from others among thick-peel melons. And then hetaomi,huanghou ,and the rest melons were classified into sub-groups. This result approached the classification of the traditional method according to characters in field.