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| 食蟹猴耳部成纤维细胞体外培养体系的初步建立 | |
| 引用本文： | 许丹娜,许惠艳,凌泽继,卢晟盛,谢莉萍,卢克焕. 食蟹猴耳部成纤维细胞体外培养体系的初步建立[J]. 安徽农业大学学报, 2009, 36(3) |
| 作者姓名： | 许丹娜 许惠艳 凌泽继 卢晟盛 谢莉萍 卢克焕 |
| 作者单位： | 1. 广西大学广西亚热带生物资源保护利用重点实验室,南宁530004;广西大学动物科技学院,南宁530004 2. 广西大学动物科技学院,南宁,530004 3. 广西玮美生物科技有限公司,南宁,530510 |
| 基金项目： | 国家自然科学基金项目(30860039);;广西科学研究与技术开发计划项目(桂科能0815011-6-3);;广西亚热带生物资源保护利用重点实验室开放课题项目(SB0701)共同资助 |
| 摘 要： | 建立食蟹猴耳部成纤维细胞的体外培养体系,为食蟹猴体细胞核移植、转基因技术及治疗性克隆做准备.通过组织块培养法进行原代培养,成功分离了食蟹猴耳部成纤维细胞;原代培养后用0.25%胰蛋白酶+0.02%EDTA消化液消化细胞,用含10%FBS的DMEM对细胞进行培养,细胞在体外传至第25代;用含10%FBS和10%DMSO的DMEM为冷冻液对细胞进行冷冻保存,解冻传代后,细胞形态和生长速度没有明显变化;用细胞技术法绘制细胞的生长曲线显示,符合体外细胞生长规律;用吉姆萨染色法对不同代数细胞的染色体倍性进行分析,二倍体细胞所占比例为80%以上. |
| 关 键 词： | 食蟹猴 耳部成纤维细胞 体外培养 |
In vitro cultivation of ear fibroblast cells from Macaca fascicularis |
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| XU Dan-n,XU Hui-yan,LING Ze-ji,LU Sheng-sheng,XIE Li-ping,LU Ke-huan. In vitro cultivation of ear fibroblast cells from Macaca fascicularis[J]. Journal of Anhui Agricultural University, 2009, 36(3) | |
| Authors: | XU Dan-n XU Hui-yan LING Ze-ji LU Sheng-sheng XIE Li-ping LU Ke-huan |
| Affiliation: | 1.Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization;Guangxi University;Nanning 530004;2.College of Animal Science and Technology;3.Guangxi Weimei Bio-tech Co.Ltd.;Nanning 530510 |
| Abstract: | In this paper,we aimed to establish an isolation method and culture system for Macaca fascicularis ear fibroblast cells in vitro by means of tissue block culture.Primary culture of Macaca fascicularis ear fibroblast cells could be obtained through tissue block culture successfully.Using 0.25% trypsin+0.02% EDTA as digested medium and DMEM containing 10% FBS as culture medium for subculture,the cells were passaged to the 25th passage,and then were frozen in DMEM medium containing 10% FBS and 10% DMSO with th... |
| Keywords: | Macaca fascicularis ear fibroblast cells in vitro culture |
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