一株类NADC30猪繁殖与呼吸综合征病毒的分离及遗传变异分析

为了解广西地区的猪繁殖与呼吸综合征病毒(PRRSV)的遗传变异及流行情况,本实验室从广西某发病猪场采集到的猪肺脏组织中检测到1株PRRSV,命名为GXNN1839,并对该毒株进行病毒的分离鉴定、GP5和Nsp2的测序分析.结果 显示:该毒株可在PAM细胞上分离增殖,有明显的细胞病变,通过IFA试验可以检测到细胞内PRR...

Genetic Variation Analysis of a NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus

To understand the genetic variation and prevalence of Porcine reproductive and respiratory syndrome virus (PRRSV) in Guangxi province,a PRRSV strain GXNN1839 was isolated from lung tissues collected from a local pig farm.The GXNN1839 strain was then sequenced and analyzed for genetic variations of GP5 and Nsp2.The results showed that the GXNN1839 strain replicated in porcine alveolar macrophages (PAMs) with obvious cytopathic effect.The expression of PRRSV N protein in PAMs was detected by IFA.The GP5 gene sequencing and genetic evolution analysis showed that this strain was classified in North American PRRSV genotype and on the same branch as the US strain NADC30.The sequence analysis showed that GP5 gene of the GXNN1839 strain had 93.1% nucleotide homology with the American NADC30 strain,91.7% with Chinese isolated NADC30-like strain CHsx1401,87% with VR-2332,86.7% with CH-1a,86.7% with the highly pathogenic strain JXA1 and 62.0% with the European Lelystad virus.In addition,the GXNN1839 strain had 131 amino acid discontinuous deletions (111+1+19) of Nsp2,identical to the North American strain NADC30.The isolation and characterization of the GXNN1839 strain provided a reference for future study on the pathogenicity of NADC30-like virus,understanding the genetic variation and prevention and control measurements of PRRS in Guangxi.