柔嫩艾美耳球虫盐霉素耐药株的实验室诱导

为了研究耐药性柔嫩艾美耳球虫产生耐药性的机理,本研究在实验室条件下,采用药物浓度递增的方法,以15 mg/kg为起始诱导浓度,对柔嫩艾美耳球虫盐霉素耐药性进行诱导。经过10次传代,获得了对60 mg/kg盐霉素耐药的柔嫩艾美耳球虫虫株。以抗球虫指数、最适抗球虫活性百分率、病变记分减少率和相对卵囊产量四项指标综合判定柔嫩艾美耳球虫对盐霉素的耐药性。其中抗球虫指数为156.92,最适抗球虫活性百分率为32.38,病变记分减少率为53.85,相对卵囊产量为28.17。结果显示,成功获得了柔嫩艾美耳球虫对盐霉素完全耐药的虫株,为进一步从分子水平研究球虫抗药性奠定基础。

Experimental Development of an Eimeria Tenella Resistant Strain to Salinomycin

An Eimeria tenella strain resistant to the anticoccidial drug salinomycin was induced experimentally in the present study.The initial concentration of salinomycin was 15 mg/kg,which was reduced during passages.As a result,an E.tenella.strain resistant to 60 mg/kg salinomycin was obtained after 10 passages in chickens.Then this drug resistant E.tenella strain was assessed in antibiotic susceptibility testing for four common anticoccidial efficacy indexes including anticoccidial index(ACI),optimal anticoccidial activity percentage(POAA),lesion score reduction rate(RLS)and relative oocyst production(ROP).The results showed anticoccidial index(ACI)at 156.92,the optimal anticoccidial activity percentage(POAA)at 32.38,the lesion score reduction rate(RLS)at 53.85,and the relative oocyst yield(ROP)at 28.17.In conclusion,a drug-resistant E.tenella strain was obtained successfully and the molecular mechanism for salinomycin resistance needs to be investigated in the future study.