Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation |
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Authors: | Ana Isabel López-Sesé Jack Staub Nurit Katzir María Luisa Gómez-Guillamón |
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Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, Vegetable Crops Unit, Department of Horticulture, University of Wisconsin Madison, 1575 Linden Dr., WI, 53706, U.S.A;(2) Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, P.O.B. 1021, Ramat Yishay, 30095, Israel;(3) Experimental Station `La Mayora', Consejo Superior de Investigaciones Científicas (CSIC), 29750 Algarrobo (Málaga), Spain |
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Abstract: | The population structure of 15 Spanish melon (C. melo L.)accessions, mostly of Group Inodorus, was assessed by the analysis of 16individuals of each accession using 100 random
amplified polymorphic DNA (RAPD) bands produced by 36 primers, and allelic variation at 12microsatellite (SSR) loci (23 alleles).
A relatively high level of polymorphism (25.6%) was detected using RAPD markers, and eight SSR loci (66.7%) were useful in
discriminating accessions. Cluster analysis using RAPD- and SSR-based genetic distance estimates resulted in similar and consistent
groupings of most of the accessions studied. The mean genetic distance and standard error among accessions estimated by RAPD
variation was 0.421 ± 0.099, and mean SSR-based genetic distance estimate was 0.285 ± 0.141. Albeit many dominant markers
examined were fitted to a 3:1 test ratio, deviation from this ratio and from Hardy-Weinberg expectations for many SSR loci
suggests that some populations were in genotypic disequilibrium. Moreover, a higher level of genetic variation was observed
between Cassaba market classes than within accessions, suggesting that, depending upon the accession, allelic fixation has
occurred in these populations. The relatively high level of heterogeneity observed (different band morphotypes and cluster
grouping within a particular market class), however, indicates that the Spanish melons examined possess a relatively broad
genetic background. An appraisal of accession population structure such as the one reported herein indicates that bulk sampling
techniques coupled with molecular analysis techniques that employ a unique array of discriminating markers can provide information
leading to effective strategies for diversity analyses of large collections.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | diversity molecular analysis genetic distance germplasmmanagement melon DNA RAPD SSR |
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