基于Nafion/天青A/纳米金层层自组装固定酶的过氧化氢生物传感器

目的]明确基于Nafion、天青A、纳米金层层自组装固定酶制备过氧化氢生物传感器的可行性。方法]先利用静电吸附和自组装技术将Nafion、天青A、纳米金修饰到金电极表面,然后利用纳米金吸附辣根过氧化物酶(HRP),制备过氧化氢生物传感器。采用循环伏安法和计时电流法考察该传感器的性能。结果]在测试溶液pH值5.5、工作电位-0.3~-0.2 V、扫速100 mV/s、检测温度25℃条件下,该传感器具有良好的生物催化活性,还原峰电流与H2O2浓度在2.0×10-6~1.6×10-3mol/L范围内呈良好的线性关系,检出限为7.62×10-7mol/L。该传感器稳定性好,抗干扰力强,制备方法简单,成本低廉,对H2O2有快速灵敏的响应。结论]该研究制备的过氧化氢生物传感器灵敏度较高,检测范围宽,检出限较低。

Hydrogen Peroxide Biosensor Based on the Layer-by-layer Self-assembly Immobilized Enzyme of Nafion,Azure A,Nano-gold

Objective] The research aimed to confirm the feasibility of preparing hydrogen peroxide biosensor based on layer-by-layer self-assembly immobilized enzyme of Nafion,azure A,nano-gold.Method] Nafion,azure A and nano-gold were decorated on the golden electrode surface by using electrostatic adsorption and self-assembly technology.Horseradish peroxidase was adsorbed by using nano-gold to prepare hydrogen peroxide biosensor.The performances of this sensor were observed by using cyclic voltammetry method and chronoamperometry.Result] Under the conditions that pH value of test solution was 5.5,working potential was-0.3～-0.2 V,sweep speed was 100 mV/s and the detection temperature was 25 ℃,this sensor had good biology catalytic activity.The reduction peak current showed a good linear correlation with H2O2 concn.in the range of 2.0×10-6～1.6×10-3 mol/L and the detection limit was 7.62×10-7 mol/L.This sensor had good stability,strong anti-disturbance capability,simple preparing method,cheap cost and rapid and sensitive response to H2O2.Conclusion] The prepared hydrogen peroxide biosensor had higher sensitivity,broad detection range and lower detection limit.