钙对化学诱抗剂诱导番茄叶片木质素合成的影响

为探索钙对化学诱抗剂水杨酸（SA）、 茉莉酸甲酯（MeJA）、 龙胆酸（GA）和 -氨基丁酸（BABA）诱导番茄木质素合成的影响，在番茄六叶期用上述诱抗剂分别处理第3 叶片，然后喷施CaCl2、 蒸馏水、 乙二醇-双-(2-氨基乙醚)四乙酸（EGTA）或LaCl3，处理后0、 1、 2、 3、 5 d 测定各处理番茄第3 叶片（诱导叶）和第5 叶片（非诱导叶）中木质素含量的变化。结果表明, （1）用上述化学诱抗剂处理番茄第3 叶片，诱导叶及其上位非诱导叶中木质素含量均升高；（2）外源Ca2+ 进一步促进上述诱抗剂诱导番茄叶片中木质素含量的增加；而Ca2+ 螯合剂EGTA和质膜钙通道抑制剂LaCl3，则不同程度地抑制这些诱抗剂对木质素含量的诱导作用。这些结果表明，钙对上述4 种诱抗剂诱导番茄叶片中木质素的合成具有正调控作用。

Effect of calcium on lignin synthesis induced by chemical elicitors

The effects of calcium on synthesis of lignin induced by chemical elicitors in tomato were investigated in this study. The 3rd leaves were smeared with chemical elicitors: salicylic acid(SA), Methyl jasmonate(MeJA), -aminobutyric acid(BABA) and gentisic acid(GA) separately at the six leaf stage of tomato. After the third leaves dried under room temperature, all the tomato plants were sprayed with CaCl2, distilled water, EGTA and LaCl3 in turn. The contents of lignin in the third leaves(induced) and the fifth leaves(un-induced) were determined in 0, 1, 2, 3 and 5 days after treatments. The results show that: (1) The lignin content in both the induced leaves and un-induced leaves increased after treated with chemical elicitors. (2) The lignin synthesis in treated tomato leaves were further promoted by exterior Ca2+, but inhibited by Ca2+ chelator EGTA and the plasmalemma Ca2+ channel blocker La3 + in different levels. These results indicated that Ca2+ play a positive regulation role in synthesis of lignin in tomato leaves induced by the above four chemical elicitors.