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| 微卫星产物变性与非变性PAGE-银染方法比较 | |
| 引用本文： | 张春雷,佟广香,匡友谊,张超,尹家胜.微卫星产物变性与非变性PAGE-银染方法比较[J].水产学杂志,2010,23(1):11-14. |
| 作者姓名： | 张春雷 佟广香 匡友谊 张超 尹家胜 |
| 作者单位： | 1. 云南农业大学动物科学技术学院,云南,昆明,650201;中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 2. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 3. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;上海海洋大学水产与生命学院,上海,201306 |
| 基金项目： | 国家科技部支撑计划资助项，黑龙江水产研究所基本科研专项资金资助项目 |
| 摘 要： | 用哲罗鱼的微卫星引物对哲罗鱼的2个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶(PAGE)上电泳,银染后发现微卫星扩增产物在二者上的电泳结果存在明显差异。在非变性凝胶中存在较多非特异条带,而在变性凝胶中微卫星扩增产物条带清晰,易于鉴定。 |
| 关 键 词： | 微卫星 聚丙烯酰胺凝胶电泳 银染 |
The Electrophoresis Comparison of Microsatellite Product Using Denaturing and Novel PAGE-Silver-Staining Methods |
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| ZHANG Chun-lei,TONG Guang-xiang,KUANG You-yi,ZHANG Chao,YIN Jia-sheng.The Electrophoresis Comparison of Microsatellite Product Using Denaturing and Novel PAGE-Silver-Staining Methods[J].Chinese Journal of Fisheries,2010,23(1):11-14. | |
| Authors: | ZHANG Chun-lei TONG Guang-xiang KUANG You-yi ZHANG Chao YIN Jia-sheng |
| Institution: | ZHANG Chun-lei1,2,TONG Guang-xiang2,KUANG You-yi2,ZHANG Chao2,3,YIN Jia-sheng2 (1. College of Animal Science , Technology,Yunnan Agriculture University,Kunming 650201,China,2. Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,3. College of Fishery , Life Science,Shanghai Ocean University,Shanghai 201306,China) |
| Abstract: | Two microsatellite primer pairs were used to amplify the genomic DNAs of hucho taimen.The PCR products were detected bydenaturingand novelpolyacrylamide gelelectrophoresis (PAGE). The resultsofsilver-staining indicated that there were manynon-spe- cific bandsproduced usingnovelgel, on the contrary, the clearlytargetbandscould be detected easilyusingdenaturinggel. |
| Keywords: | microsatellite PAGE silver-staining |
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