Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips |
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Authors: | Giuseppe Barraco Isabelle Sylvestre Florent Engelmann |
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Institution: | 1. IRD, UMR DIADE, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France;2. Università degli Studi di Palermo, Facoltà di Agraria, Viale delle Scienze ed. 4, 90128 Palermo, Italy;3. Bioversity International, Via dei Tre Denari 472/a, 00057 Maccarese (Fiumicino), Rome, Italy |
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Abstract: | In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33–37% for clone H70-144 and 20–27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 24 h in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. With both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct. |
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Keywords: | Sugarcane Cryopreservation Encapsulation-dehydration Droplet-vitrification |
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