Medium-term conservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro shoot cultures and encapsulated buds |
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| Authors: | EA Ozudogru E Kirdok E Kaya M Capuana A De Carlo F Engelmann |
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| Institution: | 1. Gebze Institute of Technology, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul Caddesi, No 101, 41400 Gebze (Kocaeli), Turkey;2. IGV/Istituto di Genetica Vegetale, CNR/Consiglio Nazionale delle Ricerche, Polo Scientifico, via Madonna del Piano 10, 50019 Sesto Fiorentino (Firenze), Italy;3. IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, CNR/Consiglio Nazionale delle Ricerche, Polo Scientifico, via Madonna del Piano 10, 50019 Sesto Fiorentino (Firenze), Italy;4. IRD/Institut de Recherche pour le Développement, UMR DIAPC, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France;5. Bioversity International, Via dei Tre Denari 472/a, 00057 Maccarese (Fiumicino), Rome, Italy |
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| Abstract: | This study evaluated the survival and recovery of non-encapsulated and encapsulated shoots of Sequoia sempervirens after storage at 4 °C in the dark for up to 15 months on four different culture media. Survival and regrowth of encapsulated shoots declined within 3 months, regardless of the storage medium composition. By contrast, no significant decrease in survival and regrowth was noted with non-encapsulated shoots after 12 months of storage on Quoirin and Lepoivre medium supplemented, or not, with 1 mg l−1 benzyladenine. Regrowth dropped to 60–61% after 15 months of storage on the same media. Medium-term conservation of S. sempervirens germplasm is therefore possible using in vitro storage of non-encapsulated shoot cultures. |
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| Keywords: | BA N6-benzyladenine HCl hydrochloric acid MS Murashige and Skoog medium NaCl sodium hypochlorite QL Quoirin and Lepoivre medium |
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