Cross-amplification of SSR markers developed from Allium sativum to other Allium species |
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Authors: | Gi-An Lee Soon-Jae Kwon Yong-Jin Park Myung-Chul Lee Haeng-Hoon Kim Jae-Sun Lee Sok-Young Lee Jae-Gyun Gwag Chung-Kon Kim Kyung-Ho Ma |
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Institution: | 1. National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, 88-20, Seodun-Dong, Suwon, Gyunggi-do 441-707, Republic of Korea;2. US Department of Agriculture-Agricultural Research Service, Western Regional Plant Introduction Station, 59 Johnson Hall, Washington State University, Pullman, WA 99164, United States;3. Department of Plant Resources, Kongju National University, Yesan 340-702, Republic of Korea;4. Garlic Research Institute, Agriculture Research and Extension Service, 659-1, Danyang 395-841, Republic of Korea |
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Abstract: | For genetic analysis of the genus Allium, which is composed of diverse species, we acquired 50 transferable and polymorphic microsatellite markers from A. sativum and tested them for transferability in five Allium species. Among the 50 simple sequence repeat (SSR) loci, the dinucleotide motif was the most prevalent, with a ratio of 50% (25/50), and (GT)n was more frequent than (GA)n within the dinucleotide motif. The average number of amplified alleles ranged from 1.452 to 1.910 and the accessions of A. tuberosum had a maximum of 4.8 alleles per accession with the GB-AS-104 SSR marker. Whereas A. porrum belonging to the Allium section revealed 73.0% transferability, A. altaicum and A. fistulosum appertaining to different sections showed low transferability, with a ratio of 47.6% and 48.0%, respectively. The phylogenetic results for these SSR markers did not deviate from previous classifications of the genus Allium. As the rate of successful amplification of SSR markers generally correlates with genetic distance, these SSR markers are potentially useful in the analysis of genetic relationships between or within Allium species. |
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Keywords: | SSR simple sequence repeat or microsatellite |
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