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Antioxidant potency of white (Brassica oleracea L. var. capitata) and Chinese (Brassica rapa L. var. pekinensis (Lour.)) cabbage: The influence of development stage,cultivar choice and seed selection
Authors:Dunja &Scaron  amec,Jasenka Piljac-Žegarac,Mara Bogović,Ksenija Habjanič,Jiří   Grú  z
Affiliation:1. Department of molecular biology, Institute Ru?er Boškovi?, Bijeni?ka c. 54, PO Box 180, 10000 Zagreb, Croatia;2. Croatian Agricultural Extension Institute, Trakoš?anska 24, 42000 Vara?din, Croatia;3. Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;4. Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany ASCR, Šlechtitel? 11, CZ-783 71 Olomouc, Czech Republic
Abstract:The accumulation of total phenols (TP, Folin-Ciocalteu method) and total flavonoids (TF, colorimetric assay with AlCl3) and the evolution of antioxidant capacity (FRAP assay, DPPH and ABTS radical scavenging assays) have been monitored in juices of Croatian white cabbage (Brassica oleracea var. capitata) cultivars Vara?dinski and Ogulinski, as well as Chinese cabbage (Brassica rapa var. pekinensis), at various developmental stages. Measurements were performed every four weeks, starting from planting to full maturity, throughout the course of eight months. In the first 8–12 weeks, the TP and TF contents as well as antioxidant capacity increased significantly in all analyzed juice samples and in most even doubled. This rapid increase was followed by a gradual decrease in values derived from all assays, over the course of 12–30 weeks, to the final values which were in all cases lower than the values measured at week 4. The results also point to significant variability in TP and TF contents and antioxidant capacity at the fully mature stage between white and Chinese cabbage juices and between juices extracted from cultivars Ogulinski and Vara?dinski.
Keywords:ABTS, 2,2&prime  -azinobis (3-ethylbenz-thiazoline-6-sulphonic acid)   CE, catechin equivalents   CHIN, Chinese cabbage   DMSO, dimethyl sulfoxide   DPPH, 2,2-diphenyl-1-picrylhydrazyl   FRAP, ferric reducing/antioxidant power   GAE, gallic acid equivalents   TF, total flavonoids   TP, total phenols   TEAC, trolox equivalent antioxidant capacity   V?-DOM, white cabbage cultivar Vara?dinski seed producer from Istria   V?-EU, white cabbage cultivar Vara?dinski seed producer from Italy   V?-LO, white cabbage cultivar Vara?dinski local seed producer   OG, white cabbage cultivar Ogulinski
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