Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran |
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| Authors: | Reza Ghanbarpour Nasrin Askari Masoud Ghorbanpour Yahya Tahamtan Khoobyar Mashayekhi Narjes Afsharipour Nasim Darijani |
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| Affiliation: | 1.Molecular Microbiology Research Group, Shahid Bahonar University of Kerman,Kerman,Iran;2.Department of Pathobiology, Faculty of Veterinary Medicine,Shahid Bahonar University of Kerman,Kerman,Iran;3.Department of Pathobiology, Faculty of Veterinary Medicine,Shahid Chamran University of Ahvaz,Ahvaz,Iran;4.Razi Vaccine and Serum Research Institute,Shiraz,Iran;5.South of Kerman Provincial Veterinary Service,Kerman,Iran |
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| Abstract: | The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests. The pathotype determination was done according to the presence of genes including f5, f41, LTI, STI, bfp, ipaH, stx 1 , stx 2 , eae, ehlyA, cnf 1 , cnf 2 , cdIII, cdIV, and f17 by PCR method. Sixty-six isolates (23.72%) possessed the STI gene and categorized into entrotoxigenic E. coli (ETEC). Nine isolates (3.1%) and five isolates (1.72%) were positive for the cnf1 and cnf2 genes which categorized into necrotoxic E. coli (NTEC). Hundred and seventeen isolates (40.34%) harbored stx 1 and/or stx 2 and classified as Shiga toxin-producing E. coli (STEC). Thirteen isolates (4.48%) were assigned to atypical entropathogenic E. coli (aEPEC) and possessed eae gene. Two isolates (0.68%) were positive for ipaH gene and were assigned to entroinvasive E. coli (EIEC). Statistical analysis showed a specific association between eae gene and STEC pathotype (P?
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