转反义ACC合成酶基因番茄与普通番茄果实植物内源激素含量的变化

 转反义 ACS( ACC合成酶基因 )番茄与普通番茄果实激素平衡方式不同。普通丽春番茄果实采前 IAA、Z+ ZR(玉米素 +玉米素核苷 )逐渐下降 ,GA在花后 40 d出现高峰 ,之后逐渐下降 ;ABA呈递增趋势 ;IAA/ ABA花后 2 0 d最高 ,花后 40 d至果实变色期变化不显著 ,但果实粉红期至红色期 IAA/ ABA显著下降。转反义 ACS番茄表现了与丽春番茄不同的变化趋势 ,其IAA/ ABA从花后 2 0 d至绿熟期呈上升趋势 ,而绿熟期至腐败期缓慢下降。转反义 ACS番茄生长类激素的含量在果实生长发育时期 (绿熟期之前 )与普通番茄没有显著差异 ,但在成熟衰老时期显著地高于普通番茄。

The Content of Hormones in Antisense ACS Tomato as Compared with Tomato cv. Lichun

The study showed that hormone balance was quite important for fruit development.The contents of IAA, GA, Z ZT were at high level at early stage of Lichun before harvest, then declined with fruit ripening , whilst ABA and ethylene increased. Because of no ethylene formation, ACS tomato reflected different situation of the content of IAA, ABA, GA, and Z ZR,but there was no significant difference in the content of growth hormone ( the total content of IAA, ABA, GA, and Z ZR)at growth period between the two varieties of tomato, while there was a significant difference at ripe ning stage. The ratio of IAA/ABA in Lichun declined at growth stage and from TU to RED stage,and if kept the same level from 40d to TU. But the ratio of IAA/ABA in ACS tomato increased at growth stage and then decreased after harvest. Treated with 0.02 ethrel, the content of GA and Z ZR in antisense ACS tomato fruit declined significantly, but the content of ABA increased significantly and the content of IAA did not change much.