| 裕丹参不同变异类型ISSR鉴定 |
| |
| 引用本文： | 高致明,孙寒,吴月红,张红瑞. 裕丹参不同变异类型ISSR鉴定[J]. 河南农业大学学报, 2010, 44(2) |
| |
| 作者姓名： | 高致明 孙寒 吴月红 张红瑞 |
| |
| 作者单位： | 河南农业大学农学院,河南,郑州,450002;河南农业大学农学院,河南,郑州,450002;河南农业大学农学院,河南,郑州,450002;河南农业大学农学院,河南,郑州,450002 |
| |
| 基金项目： | 河南省科技重大攻关项目 |
| |
| 摘 要： | 采用ISSR分子标记对裕丹参4种变异类型基因组DNA进行PCR扩增,利用SPSS11.5软件进行类型问亲缘关系分析,构建遗传树状图.结果表明,从50务ISSR引物中筛选出8条具有鉴别意义的多态性引物,在裕丹参4种变异类型中共得到65条带,47条为多态性条带,多态性位点72.3%,聚类分析将裕丹参4种类型分为2个类群,表明裕丹参4种变异类型遗传分化明显.ISSR标记可以作为裕丹参4种变异类型鉴定的有效分子标记.
|
| 关 键 词： | 裕丹参 变异类型 微卫星间隔 |
ISSR molecular identification of the variance-types of Salvia miltiorrhiza |
| |
| GAO Zhi-ming,SUN Han,WU Yue-hong,ZHANG Hong-rui. ISSR molecular identification of the variance-types of Salvia miltiorrhiza[J]. Journal of Henan Agricultural University, 2010, 44(2) |
| |
| Authors: | GAO Zhi-ming SUN Han WU Yue-hong ZHANG Hong-rui |
| |
| Affiliation: | College of Agronomy/a>;Henan Agricultural University/a>;Zhengzhou 450002/a>;China |
| |
| Abstract: | PCR amplification was performed with the genomic DNA of four variance-types of Salvia miltiorrhiza by ISSR molecular markers.The genetic relationships were analyzed by using analysis software SPSS11.5,and the DNA molecular dendrogram was established.The results showed that eight polymorphic primers for identification were chosen from 50 ISSR primers.A total of 65 bands were amplified from four variance-types of S.miltiorrhiza using the eight screened primers,including 47 polymorphic bands taking up about 72... |
| |
| Keywords: | Salvia miltiorrhiza variance-type inter simple sequence repeat |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
