| 基于nrDNA ITS序列对枸杞雄性不育材料的鉴别 |
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| 引用本文： | 石志刚. 基于nrDNA ITS序列对枸杞雄性不育材料的鉴别[J]. 江苏农业学报, 2009, 25(2) |
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| 作者姓名： | 石志刚 |
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| 作者单位： | 宁夏枸杞工程技术研究中心,宁夏银川,750002 |
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| 摘 要： | 对7份常规枸杞种质和1份枸杞雄性不育材料的DNA中nrDNA ITS(核糖体DNA基因内转录间隔区)序列进行分析,从分子水平对枸杞雄性不育材料作出鉴定.采用改进CTAB法提取枸杞叶片DNA,利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆,对目的片段测序分析.结果显示首次测序得到了枸杞雄性不育材料和其他7份常规枸杞种质的nrDNA ITS区碱基序列,整个ITS序列长度为559～633 bp,平均为612 bp,共有160个变异位点,占25.3%;保守位点473,占74.7%;67个转换位点和31个颠换位点.表明基于nrDNA ITS区序列分析可作为鉴定枸杞雄性不育材料的一种新的鉴别方法.
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| 关 键 词： | 枸杞 ITS序列 DNA测序 鉴定 |
Identification of Male Sterility in Lycium barbarum L.Based on nrDNA ITS Sequence |
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| SHI Zhi-gang. Identification of Male Sterility in Lycium barbarum L.Based on nrDNA ITS Sequence[J]. Jiangsu Journal of Agricultural Sciences, 2009, 25(2) |
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| Authors: | SHI Zhi-gang |
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| Affiliation: | Ningxia Wolfberry Engineering and Technology Research Center;Yinchuan 750002;China |
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| Abstract: | The nrDNA ITS sequence of the male sterility YX-1 and other 7 germplasms in Lycium were analysed to identify the male sterility YX-1 at molecular level.Genomic DNA from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification,cloning and sequencing by synthetic specific primer the nrDNA ITS sequences of male sterility YX-1 and other 7 germplasms in Lycium were gained for the first time.The variation length of the whole ITS sequence was 559-633 bp and average ... |
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| Keywords: | Lycium barbarum L. ITS sequence measure ment of DNA sequence identification |
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