精油和饲用酶对肉仔鸡肠道菌群的影响

采用单因子分组试验设计,将800只1日龄AA肉仔鸡公雏随机分为5组(各8个重复,每个重复20只鸡),随机施予5个处理,即基础饲粮(B)(Ⅰ)、B+50g·t-1精油(EO)(Ⅱ)、B+100g·t-1EO(Ⅲ)、B+500g·t-1Avizyme+100g·t-1植酸酶(Ⅳ)和B+500g·t-1Avizyme+100g·t-1植酸酶+100g·t-1EO(Ⅴ).试验期(42d)中测定了粪便、回肠与盲肠内容物中3种细菌数及回盲肠内容物的挥发性脂肪酸(VFAs)浓度.结果表明:单独添加EO(Ⅱ、Ⅲ组)未显著减少仔鸡粪便、回肠与盲肠内容物大肠杆菌数(P0.05);加酶(Ⅳ组)仅在第11d的粪便中的大肠杆菌数低于其它各处理组;EO或酶对弯曲杆菌数和产气荚膜梭菌数的影响均不规律;添加EO、酶或二者合用,对盲肠内容物中VFAs浓度无显著影响(P0.05).

Effects of supplementing essential oil and enzymes in diets on intestinal microflora of broilers

This experiment was carried out to study the effects of supplementing essential oils (EO) or enzymes(Enm) in diets on laying performance and intestinal microflora of broilers. A single factorial design was used in the experiment. Eight hundred AA male broilers at one-day age were divided into 5 groups at 8 replicates and each with 20 birds. Each group was allocated randomly to one of the five dietary treatments,basal diet (B)(Ⅰ), B+50 g·t-1 EO (Ⅱ),B+100 g·t-1 EO (Ⅲ), B+500 g avizyme and 100 g·t-1 phytase(Ⅳ) and B + 500 g·t-1 avizyme+100 g·t-1 phytase+100 g·t-1 EO (Ⅴ). Numbers of three bacteria species in excrete (Ex) , contents of ileum (IC) and cecum (CC) were measured in-test of 42 days. The showed thatthe numbers of E. coli in Ex , IC and CC were not decreased by adding EO; and the number of E. coli in only the Ex of 11 d decreased by adding Enm(P<0.05). Identity of the effects added with EO or Enm on numbers of Campylobacter and Clostridium perfringens was not found. The VFAs levels of CC were not effected by adding of EO,Enm or EO+Enm.It is included that effects of adding EO on numbers of E. coli in excretes,contents of ileum and cecum were not occurred,as well VFAs levels in contents of cecum.There were not regular patterns on effects of adding EO on numbers of Campylobacter and Clostridium perfringens