深绿木霉HB20111产挥发性物质及其功能分析

为了解深绿木霉HB20111产挥发性物质的成分以及潜在的生物学功能,采用顶空气相色谱-质谱法测定了深绿木霉HB20111产挥发性物质的主要成分,并进行了成分分析;采用对扣法测定了深绿木霉HB20111产挥发性物质对5种植物病原菌的抑菌率;同时测定了深绿木霉HB20111产挥发性物质对小麦幼苗生长的影响。结果表明,深绿木霉HB20111产挥发性物质烯类的相对含量最高,为49.68%、醇类为21.78%,酮类为8.56%,其余类别相对含量较低;这些挥发性物质对5种植物病原菌均有一定的抑制效果,其中对立枯丝核菌抑菌率最高,为50.62%;挥发性物质还可以促进小麦幼苗生长,与对照相比,对小麦幼苗的株高有显著的促进作用(P<0.05)。这些结果为深绿木霉HB20111的开发应用提供了理论基础。

Identification and functional analysis of volatile organic compounds from Trichoderma atroviride HB20111

In order to determine the components and potential biological functions of volatile organic compounds (VOCs) produced by Trichoderma atroviride HB20111, the headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to analyze the VOCs from T.atroviride HB20111. The inhibition rate of VOCs against five plant pathogenic fungi was measured by using plate-to-plate method, and the promoting effect of the VOCs on the growth of wheat seedlings was explored. The results showed that there were three major classes of VOCs, including alkenes,alcohols and ketones, accounting for 49.68%, 21.78% and 8.56% of the total amount, respectively. These VOCs had certain inhibitory effects on the five plant pathogens, and the highest inhibition rate was 50.62% against Rhizoctonia solani. The VOCs also promoted the growth of wheat seedlings and significantly promoted the plant height of wheat seedlings (P<0.05) compared with the control. These results provide a basis for further application of T.atroviride HB20111.