胡杨根际可培养细菌的分离及抑制瓜列当种子萌发研究

从胡杨生存的干旱强光照逆境中获得新的细菌资源,从中筛选新的可抑制瓜列当种子萌发的生防菌。采用3种分离培养基,以稀释涂布法对塔里木河流域3处胡杨根系样品进行可培养细菌分离,通过分析分离菌株16S rRNA基因序列确定其种属分类。采用皿内培养法测定其对瓜列当种子萌发的抑制活性,利用盆栽试验检测分离菌株M2-3对瓜列当寄生的防治效果。结果分离获得98株细菌,分属于28个属,马赛菌属Massilia、Pontibacter、玫瑰单胞菌属Roseomonas为优势菌属。其中,菌株M2-3与Pontibacter salisaro HMC5104T的相似度最高,为97.92%,其菌体发酵液10倍稀释液对瓜列当种子萌发抑制率为87.8%,盆栽试验表明该菌株对瓜列当防效可达36.9%,具备进一步开发可能性。

Isolation of culturable bacteria from the rhizosphere of Populus euphratica and inhibition activity on the seed germination of Orobanche aegyptiaca

New bacterial resources were obtained from the drought and high light environment where Populus euphratica grows and new biocontrol resources for inhibiting the seed germination of Orobanche aegyptiaca were screened. Culturable bacteria were isolated from three P.euphratica root samples in Tarim River basin by dilution plating method and identified by 16S rRNA gene sequence analysis. In vitro culture method was used to determine the inhibition activity on the seed germination of O.aegyptiaca. Pot experiment was used to detect the control effect of M2-3 against the parasitism of O.aegyptiaca. Results showed that 98 bacteria strains were isolated, belonging to 28 genera, among which Massilia, Pontibacter and Roseomonas were the dominant bacteria.The similarity between strain M2-3 and Pontibacter salisaro strain HMC5104T was 97.92%.The inhibiting rate of 10-fold diluted fermentation filtrate on seed germination of O.aegyptiaca was 87.8%, and the control efficacy was up to 36.9%. The strain M2-3 had the potential for further development.