| 大丽轮枝菌拮抗细菌HMB-1005菌株的鉴定 |
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| 引用本文： | 王世英,王占利,李术娜,姜军坡,朱宝成.大丽轮枝菌拮抗细菌HMB-1005菌株的鉴定[J].河南农业科学,2009(5). |
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| 作者姓名： | 王世英 王占利 李术娜 姜军坡 朱宝成 |
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| 作者单位： | 1. 河北农业大学,生命科学学院,河北,保定,071001
2. 保定学院,资源与环境系,河北,保定,071002 |
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| 基金项目： | 河北省自然科学基金项目 |
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| 摘 要： | 产芽孢细菌HMB-1005对棉花黄萎病病原菌大丽轮枝菌(Verticillium dahliae)有强烈拮抗作用。盆栽试验显示:HMB-1005对棉花黄萎病有较好的防效。通过对菌落菌体形态观察、生理生化试验和16S rDNA序列分析,以及对HMB-1005菌株分类地位进行鉴定。结果表明:HMB-1005菌株的菌落菌体形态及19项生理生化特征与解淀粉芽孢杆菌(Bacillus amylolique faciens)完全相符,16S rDNA序列同源相似度为99.28%,因此,可将HMB-1005菌株归为解淀粉芽孢杆菌。
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| 关 键 词： | 大丽轮枝菌 拮抗细菌 鉴定 解淀粉芽孢杆菌 |
Identification of Antagonistic Strain HMB-1005 against Verticillium dahliae |
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| WANG Shi-ying,WANG Zhan-li,LI Shu-na,JIANG Jun-po,ZHU Bao-cheng.Identification of Antagonistic Strain HMB-1005 against Verticillium dahliae[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | WANG Shi-ying WANG Zhan-li LI Shu-na JIANG Jun-po ZHU Bao-cheng |
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| Institution: | 1.College of Life Science;Agricultural University of Hebei;Baoding 071001;China;2.Department of Resource and Environment;Baoding University;Baoding 071002;China |
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| Abstract: | Antagonistic strain HMB-1005 was an antagonistic bacterium with high antagonistic activity against Verticillium dahlia.Pot experiment showed effective to Verticillium dahliae.Morphological observation,physiological and biochemical test and 16S rDNA sequence analysis determined the taxonomic position of strain HMB-1005.Characteristics of strain HMB-1005 equates well with Bacillus amyloliquefaciens at the level of species in morphological,19 physi-ological and biochemical items.Antagonistic strain HMB-1005 wa... |
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| Keywords: | Verticillium dahliae Antagonistic bacteria Identification Bacillus amyloliquefaciens |
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