| 蝇蛆生物活性肽的生产工艺研究 |
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| 引用本文： | 李玲玲,;李帅,;姜宁,;张爱忠.蝇蛆生物活性肽的生产工艺研究[J].家畜生态,2008(3):53-55. |
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| 作者姓名： | 李玲玲 ;李帅 ;姜宁 ;张爱忠 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院 黑龙江大庆163319 |
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| 基金项目： | [基金项目]黑龙江省农垦总局“十一五”科技攻关项目资助（HNKXIV-09-02-05）. |
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| 摘 要： | 以蝇蛆为试验材料，设酶解温度、复合酶量、酶配比、酶解时问和pH值5个因素，每个因素4个水平进行L15（4^5）正交试验设计，以水解度作为评价指标，筛选脱脂蝇蛆粉的最佳酶解条件。结果表明：脱脂蝇蛆粉的最优酶解条件为A1B3C3D4E3，即温度（A）45℃，酶量（B）8000U／g，风味酶：木瓜酶：胃蛋白酶：胰蛋白酶的配比（C）是35％：50％：5％：10％，酶水解时间（D）为7h，pH（E）为7．5。脱脂蝇蛆粉溶液水解度的最大影响因素为酶解温度。
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| 关 键 词： | 蝇蛆 酶解 工艺参数 |
Selection of Enzymolysis Technologic Parameter in Defatted Fly Maggot |
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| LI Ling-ling,LI Shuai,JIANG Ning,ZHANG Ai-zhong.Selection of Enzymolysis Technologic Parameter in Defatted Fly Maggot[J].Ecology of Domestic Animal,2008(3):53-55. |
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| Authors: | LI Ling-ling LI Shuai JIANG Ning ZHANG Ai-zhong |
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| Institution: | LI Ling-ling,LI Shuai,JIANG Ning,ZHANG Ai-zhong (College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China ) |
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| Abstract: | The fly maggot was used to select the optimal enzyme hydrolysis condition of defatted fly maggot. And the temperature,enzyme amount,enzyme match, time and pH serve as five factors among which each factors have four level in progress L16 （4^5）orthogonal experiment design with hydrolysis degree as assessment index. The results showed that the optimal enzymolysis condition of defatted fly maggot was A1B3C3D4E3 , which the percentage of flavor enzyme, papain, pepsin and trypsin （C） were 35%, 50%, 5% and 10% respectively, enzyme amount （B） was 8000U/g, and the temperature （A）, time （D） and pH （E） of enzymatic hydrolysis were 45℃, 7h, and 7.5 respectively. Hydrolysis temperature was the main influence factor in defatted fly maggot enzymolysis. |
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| Keywords: | fly maggot enzymolysis technologic parameter |
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